Detection of high PBDD/Fs levels and dioxin-like activity in toys using a combination of GC-HRMS, rat-based and human-based DR CALUX® reporter gene assays
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
32443226
DOI
10.1016/j.chemosphere.2020.126579
PII: S0045-6535(20)30772-4
Knihovny.cz E-resources
- Keywords
- Children’s toys, DR CALUX, Dioxins, Polybrominated dibenzo-p-dioxin and dibenzofuran (PBDD/Fs), Reporter gene assays,
- MeSH
- Biological Assay MeSH
- Cell Line MeSH
- Child MeSH
- Play and Playthings * MeSH
- Rats MeSH
- Environmental Pollutants analysis toxicity MeSH
- Humans MeSH
- Luciferases genetics MeSH
- Environmental Monitoring methods MeSH
- Plastics chemistry standards MeSH
- Gas Chromatography-Mass Spectrometry MeSH
- Polychlorinated Dibenzodioxins analysis toxicity MeSH
- Dibenzofurans, Polychlorinated analysis toxicity MeSH
- Child, Preschool MeSH
- Receptors, Aryl Hydrocarbon genetics MeSH
- Genes, Reporter MeSH
- Flame Retardants analysis toxicity MeSH
- Transfection MeSH
- Animals MeSH
- Check Tag
- Child MeSH
- Rats MeSH
- Humans MeSH
- Child, Preschool MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Environmental Pollutants MeSH
- Luciferases MeSH
- Plastics MeSH
- Polychlorinated Dibenzodioxins MeSH
- Dibenzofurans, Polychlorinated MeSH
- Receptors, Aryl Hydrocarbon MeSH
- Flame Retardants MeSH
Brominated dibenzo-p-dioxins and dibenzofurans (PBDD/Fs) are increasingly reported at significant levels in various matrices, including consumer goods that are manufactured from plastics containing certain brominated flame retardants. PBDD/Fs are known ligands for the aryl hydrocarbon receptor (AhR) but are not yet considered in the hazard assessment of dioxin mixtures. The aim of the present study was to determine if PBDD/Fs levels present in plastic constituents of toys could pose a threat to children's health. PBDD/Fs, unlike their chlorinated counterparts (PCDD/Fs), have not been officially assigned toxic equivalence factors (TEFs) by the WHO therefore, we determined their relative potency towards AhR activation in both human and rodent cell-based DR CALUX® bioassays. This allowed us to compare GC-HRMS PBDD/F congener levels, converted to total Toxic Equivalents (TEQ) by using the PCDD/F TEFs, to CALUX Bioanalytical Equivalents (BEQ) levels present in contaminated plastic constituents from children's toys. Finally, an estimate was made of the daily ingestion of TEQs from PBDD/Fs-contaminated plastic toys by child mouthing habits. It is observed that the daily ingestion of PBDD/Fs from contaminated plastic toys may significantly contribute to the total dioxin daily intake of young children.
Arnika Toxics and Waste Programme Delnicka 13 Prague Czech Republic
BioDetection Systems B 5 Science Park 406 1098XH Amsterdam the Netherlands
Mas |münsteranalytical solutions gmbh Wilhelm Schickard Strasse 5 48149 Münster Germany
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