Glucan-Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Kupffer Cells in Mouse Liver
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Diabetes, Gene silencing, Inflammation, Insulin sensitivity, Intravenous administration, Kupffer cells, Mouse liver, Obesity, RNAi delivery,
- MeSH
- Glucans genetics MeSH
- Liver physiology MeSH
- Kupffer Cells physiology MeSH
- Macrophages physiology MeSH
- RNA, Small Interfering genetics MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- RNA Interference physiology MeSH
- Gene Silencing physiology MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Glucans MeSH
- RNA, Small Interfering MeSH
Here, we describe a protocol to prepare and administer glucan-encapsulated RNAi particles (GeRPs), for specific delivery of siRNA and subsequent gene silencing in Kupffer cells (KCs) in mice. This technology is based on baker's yeast and allows gene manipulation in macrophages in a tissue-specific manner depending on the route of administration and the model that is used. GeRP administered by intravenous injection in mice are delivered to KCs. Therefore, using the GeRP technology to silence genes provides a unique method to study the function of factors expressed by KCs in the regulation of liver function.
References provided by Crossref.org
