At present, the traditional methods for the screening of Clostridium butyricum are not sufficiently selective and efficient. Therefore, it is necessary to establish a targeted and efficient screening method for the detection of C. butyricum. Bioinformatics was used in this study to find C. butyricum specific genes, and species-specific primers were designed based on the conserved regions of the targeted genes, followed by optimization of the PCR conditions. Methodological evaluation was carried out, and the results were compared with the traditional screening method based on Trypticase Sulfite Neomycin (TSN) selective medium. A high-efficiency PCR screening method, targeting C. butyricum species-specific primers, was established. The method was confirmed to have high specificity and sensitivity towards C. butyricum cut-off CFU 103. Compared with the traditional method, the screening success rate of C. butyricum strains increased from 0.61 to 81.91%. The PCR screening method could quickly and accurately detect C. butyricum in samples and dramatically improve screening efficiency.

Beijing Innovation Centre of Food Nutrition and Human Health Beijing Technology and Business University Beijing 100048 China

National Engineering Research Center for Functional Food Jiangnan University Wuxi 214122 Jiangsu Province China

School of Food Science and Technology Jiangnan University Wuxi 214122 Jiangsu Province China

State Key Laboratory of Food Science and Technology Jiangnan University Wuxi 214122 Jiangsu Province China

Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch Wuxi 214122 Jiangsu Province China

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