Phycobilisome integrity and functionality in lipid unsaturation and xanthophyll mutants in Synechocystis
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
bilateral exchange visit program between the Bulgarian and Hungarian Academy of Sciences
Bulgarian Academy of Sciences
Hungarian Government Grant GINOP-2.3.2-15-2016-00001
Hungarian Scientific Research Fund
PubMed
32720110
DOI
10.1007/s11120-020-00776-1
PII: 10.1007/s11120-020-00776-1
Knihovny.cz E-zdroje
- Klíčová slova
- Carotenoids, Cyanobacteria, Excitation energy transfer, Lipid unsaturation, Phycobilisome, Thermal stability,
- MeSH
- fotosyntetické reakční centrum - proteinové komplexy metabolismus MeSH
- fotosyntéza * MeSH
- fykobilizomy metabolismus MeSH
- fykokyanin metabolismus MeSH
- karotenoidy metabolismus MeSH
- metabolismus lipidů MeSH
- mutace MeSH
- Synechocystis genetika metabolismus MeSH
- teplota MeSH
- tylakoidy metabolismus MeSH
- xanthofyly metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fotosyntetické reakční centrum - proteinové komplexy MeSH
- fykobilizomy MeSH
- fykokyanin MeSH
- karotenoidy MeSH
- xanthofyly MeSH
The major light-harvesting system in cyanobacteria, the phycobilisome, is an essential component of the photosynthetic apparatus that regulates the utilization of the natural light source-the Sun. Earlier works revealed that the thylakoid membrane composition and its physical properties might have an important role in antennas docking. Polyunsaturated lipids and xanthophylls are among the most significant modulators of the physical properties of thylakoid membranes. In the nature, the action of these molecules is orchestrated in response to environmental stimuli among which the growth temperature is the most influential. In order to further clarify the significance of thylakoid membrane physical properties for the phycobilisomes assembly (i.e. structural integrity) and their ability to efficiently direct the excitation energy towards the photosynthetic complexes, in this work, we utilize cyanobacterial Synechocystis sp. PCC 6803 mutants deficient in polyunsaturated lipids (AD mutant) and xanthophylls (RO mutant), as well as a strain depleted of both xanthophylls and polyunsaturated lipids (ROAD multiple mutant). For the first time, we discuss the effect of those mutations on the phycobilisomes assembly, integrity and functionality at optimal (30 °C) and moderate low (25 °C) and high (35 °C) temperatures. Our results show that xanthophyll depletion exerts a much stronger effect on both phycobilisome's integrity and the response of cells to growth at suboptimal temperatures than lipid unsaturation level. The strongest effects were observed for the combined ROAD mutant, which exhibited thermally destabilized phycobilisomes and a population of energetically uncoupled phycocyanin units.
Department of Plant Biology University of Szeged Szeged Hungary
Institute of Biophysics and Biomedical Engineering Bulgarian Academy of Sciences Sofia Bulgaria
Institute of Genetics Biological Research Centre Szeged Hungary
Institute of Photonics and Electronics The Czech Academy of Sciences Prague Czech Republic
Institute of Plant Biology Biological Research Centre Szeged Hungary
Molecular Plant Biology Department of Biochemistry University of Turku Turku Finland
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