In vitro evaluation of a novel nanostructured Ti-36Nb-6Ta alloy for orthopedic applications
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
32752935
DOI
10.2217/nnm-2020-0123
Knihovny.cz E-zdroje
- Klíčová slova
- adhesion, anodization, beta titanium alloy, biocompatibility, hMSC, mechanical properties, nanotubes, osteogenic differentiation, proliferation,
- MeSH
- buněčná adheze MeSH
- lidé MeSH
- mikroskopie elektronová rastrovací MeSH
- nanotrubičky * MeSH
- osteoblasty MeSH
- osteogeneze MeSH
- povrchové vlastnosti MeSH
- proliferace buněk MeSH
- slitiny MeSH
- titan * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- slitiny MeSH
- titan * MeSH
Aim: To evaluate the impact of a nanostructured surface created on β-titanium alloy, Ti-36Nb-6Ta, on the growth and differentiation of human mesenchymal stem cells. Materials & methods: The nanotubes, with average diameters 18, 36 and 46 nm, were prepared by anodic oxidation. Morphology, hydrophilicity and mechanical properties of the nanotube layers were characterized. The biocompatibility and osteogenic potential of the nanostructured surfaces were established using various in vitro assays, scanning electron microscopy and confocal microscopy. Results: The nanotubes lowered elastic modulus close to that of bone, positively influenced cell adhesion, improved ALP activity, synthesis of type I collagen and osteocalcin expression, but diminished early cell proliferation. Conclusion: Nanostructured Ti-36Nb-6Ta with nanotube diameters 36 nm was the most promising material for bone implantation.
Citace poskytuje Crossref.org
Human cells with osteogenic potential in bone tissue research
