Vga(A) protein variants confer different levels of resistance to lincosamides, streptogramin A, and pleuromutilins (LSAP) by displacing antibiotics from the ribosome. Here, we show that expression of vga(A) variants from Staphylococcus haemolyticus is regulated by cis-regulatory RNA in response to the LSAP antibiotics by the mechanism of ribosome-mediated attenuation. The specificity of induction depends on Vga(A)-mediated resistance rather than on the sequence of the riboregulator. Fine tuning between Vga(A) activity and its expression in response to the antibiotics may contribute to the selection of more potent Vga(A) variants because newly acquired mutation can be immediately phenotypically manifested.

Department of Clinical Medicine UiT The Arctic University of Norway Tromsø Norway

Department of Genetics and Microbiology Faculty of Science Charles University Prague Czech Republic

Department of Pediatrics University Hospital of North Norway Tromsø Norway

Institute of Microbiology of the Czech Academy of Sciences Vestec Czech Republic

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Antimicrob Agents Chemother. 2020 Dec 16;65(1):e02242-20. doi: 10.1128/AAC.02242-20. PubMed

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