Pseudomonas karstica sp. nov. and Pseudomonas spelaei sp. nov., isolated from calcite moonmilk deposits from caves
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články
PubMed
32821035
DOI
10.1099/ijsem.0.004393
Knihovny.cz E-zdroje
- Klíčová slova
- Pseudomonas karstica, Pseudomonas spelaei, caves, moonmilk, sp. nov.,
- MeSH
- bakteriální geny MeSH
- DNA bakterií genetika MeSH
- fylogeneze * MeSH
- hybridizace nukleových kyselin MeSH
- jeskyně mikrobiologie MeSH
- lipidy analýza MeSH
- mastné kyseliny chemie MeSH
- Pseudomonas klasifikace MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- techniky typizace bakterií MeSH
- uhličitan vápenatý * MeSH
- zastoupení bazí MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- DNA bakterií MeSH
- lipidy MeSH
- mastné kyseliny MeSH
- RNA ribozomální 16S MeSH
- uhličitan vápenatý * MeSH
A taxonomic study of two fluorescent Pseudomonas strains (HJ/4T and SJ/9/1T) isolated from calcite moonmilk samples obtained from two caves in the Moravian Karst in the Czech Republic was carried out. Results of initial 16S rRNA gene sequence analysis assigned both strains into the genus Pseudomonas and showed Pseudomonas yamanorum 8H1T as their closest neighbour with 99.8 and 99.7 % 16S rRNA gene similarities to strains HJ/4T and SJ/9/1T, respectively. Subsequent sequence analysis of rpoD, rpoB and gyrB housekeeping genes confirmed the highest similarity of both isolates to P. yamanorum 8H1T, but phylogeny and sequences similarities implied that they are representatives of two novel species within the genus Pseudomonas. Further study comprising whole-genome sequencing followed by average nucleotide identity and digital DNA-DNA hybridization calculations, repetitive sequence-based PCR fingerprinting with the REP and ERIC primers, automated ribotyping with the EcoRI restriction endonuclease, cellular fatty acid analysis, quinone and polar lipid characterization, and extensive biotyping confirmed clear separation of both analysed strains from the remaining Pseudomonas species and showed that they represent two novel species within the genus Pseudomonas for which the names Pseudomonas karstica sp. nov. (type strain HJ/4T=CCM 7891T=LMG 27930T) and Pseudomonas spelaei sp. nov. (type strain SJ/9/1T=CCM 7893T=LMG 27931T) are suggested.
Centre for Cardiovascular Surgery and Transplantation Pekařská 53 656 91 Brno Czech Republic
PhiXGen Pvt Ltd 101 GH 11 Atlantis CGHS Ltd Sector 47 Gurugram Haryana 122001 India
The Energy and Resources Institute Lodhi Road New Delhi 110003 India
Citace poskytuje Crossref.org
Environmental Drivers of the Moonmilk Microbiome Diversity in Some Temperate and Tropical Caves
