Monolithic Poly(styrene-co-divinylbenzene) Columns for Supercritical Fluid Chromatography-Mass Spectrometry Analysis of Polypeptide
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- biosenzitivní techniky MeSH
- gramicidin analýza izolace a purifikace MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací MeSH
- methanol chemie MeSH
- molekulová hmotnost MeSH
- oxid uhličitý chemie MeSH
- polystyreny chemie MeSH
- poréznost MeSH
- protein - isoformy chemie MeSH
- superkritická fluidní chromatografie metody MeSH
- teplota MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- divinylbenzene-polystyrene copolymer MeSH Prohlížeč
- gramicidin MeSH
- methanol MeSH
- oxid uhličitý MeSH
- polystyreny MeSH
- protein - isoformy MeSH
The organic polymer-based monolithic columns have been evaluated as the separation media for analysis of peptides using supercritical fluid chromatography-mass spectrometry (SFC-MS). We demonstrate for the first time the SFC-MS separation of a mixture of polypeptides carried out using poly(styrene-co-divinylbenzene) monolithic columns and carbon dioxide/methanol mobile phase. A gradient from 2 to 40% methanol modifier containing 0.1% TFA as an acidic additive was applied for the optimized elution and the separation was achieved in less than 3 min. Selected ion monitoring enabled detection of selected masses characteristic of three ionophoric pentadecapeptide antibiotics gramicidin A, B, and C and their two corresponding isoforms. Furthermore, their identity was confirmed through determination of their [M + 2H]2+, [M + 2Na]2+, and [M + H + Na]2+ ions acquired by positive-ion electrospray ionization-mass spectrometry (ESI-MS).
Citace poskytuje Crossref.org
Recent developments and applications of polymer monolithic stationary phases
