Substitution-Inert Polynuclear Platinum Complexes Inhibit Reverse Transcription Preferentially in RNA Triplex-Forming Templates
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
- MeSH
- komplexní sloučeniny chemie MeSH
- konformace nukleové kyseliny MeSH
- platina chemie MeSH
- protinádorové látky chemie MeSH
- reverzní transkripce účinky léků MeSH
- RNA chemie MeSH
- sekvence nukleotidů MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- komplexní sloučeniny MeSH
- platina MeSH
- protinádorové látky MeSH
- RNA MeSH
RNA triplexes are significant tertiary structure motifs that are found in many functional RNAs. Hence, small molecules capable of recognition, binding, and stabilization of the triple-helical RNA structures are emerging as attractive potential molecular biology tools and therapeutic agents. Here, we utilize methods of molecular biology and biophysics to study the interactions of a series of antitumor substitution-inert polynuclear platinum complexes (SI-PPCs) with triple-helical RNA structures. We show that SI-PPCs recognize and stabilize RNA triplexes and inhibit reverse transcription preferentially in the RNA template prone to the triplex formation. These so far unexplored properties of SI-PPCs suggest that the targeting of triple-stranded regions in RNA might contribute to the biological effects of SI-PPCs.
Czech Academy of Sciences Institute of Biophysics Kralovopolska 135 Brno CZ 61265 Czech Republic
Department of Chemistry Virginia Commonwealth University Richmond Virginia 23284 2006 United States
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