Wild boar as a potential reservoir of zoonotic tick-borne pathogens
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
33010631
DOI
10.1016/j.ttbdis.2020.101558
PII: S1877-959X(20)30427-1
Knihovny.cz E-zdroje
- Klíčová slova
- Anaplasma phagocytophilum, Ixodesricinus, Piroplasmids, Wild boar, Zoonosis,
- MeSH
- Anaplasma phagocytophilum genetika izolace a purifikace MeSH
- anaplasmóza epidemiologie mikrobiologie MeSH
- babezióza epidemiologie parazitologie MeSH
- bakteriální geny MeSH
- genetická variace * MeSH
- nemoci prasat epidemiologie mikrobiologie parazitologie MeSH
- Piroplasmida genetika izolace a purifikace MeSH
- prasata MeSH
- prevalence MeSH
- protozoální geny MeSH
- Sus scrofa MeSH
- zdroje nemoci parazitologie veterinární MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
The wild boar (Sus scrofa) population has increased dramatically over the last decades throughout Europe and it has become a serious pest. In addition, the common habitat of wild boar and of the tick, Ixodes ricinus, indicates the potential of wild boar to play a role in epidemiology of epizootic and zoonotic tick-borne pathogens, including Anaplasma phagocytophilum. In Europe, epidemiological cycles and reservoirs of A. phagocytophilum, including its zoonotic haplotypes, are poorly understood. In this study, we focused on detection and further genetic characterization of A. phagocytophilum and piroplasmids in 550 wild boars from eleven districts of Moravia and Silesia in the Czech Republic. Using highly sensitive nested PCR targeting the groEL gene, the DNA of A. phagocytophilum was detected in 28 wild boars (5.1 %) representing six unique haplotypes. The dominant haplotype was found in 21 samples from 7 different districts. All detected haplotypes clustered in the largest clade representing the European ecotype I and the dominant haplotype fell to the subclade with the European human cases and strains from dogs and horses. Nested PCR targeting the variable region of the 18S rRNA gene of piroplasmids resulted in one positive sample with 99.8 % sequence identity to Babesia divergens. The presence of these two pathogens that are primarily circulated by I. ricinus confirms the local participation of wild boar in the host spectrum of this tick and warrants experimental studies to address wild boar as a reservoir of zoonotic haplotypes of A. phagocytophilum.
State Veterinary Institute Jihlava Rantířovská 93 20 Horní Kosov 58601 Jihlava Czech Republic
State Veterinary Institute Olomouc Jakoubka ze Stříbra No 1 779 00 Olomouc Czech Republic
Citace poskytuje Crossref.org
