Mechanically separated fish meat (MSFM) can be used for the manufacturing of ready-to-eat products, such as sausages; however, it is highly perishable. Several plant by-products, including onion peel, which is rich in polyphenol antioxidants, can be added to food to extend shelf life. This study investigated the effects of the addition of onion peel powder (OPP) to sausage made from MSFM. Sausages were divided into four groups with different amounts of added OPP: 0% (control), 1%, 2%, and 3%. Cooked sausages were stored for 28 days at 5 °C. Samples were analyzed for thiobarbituric acid reactive substances, antioxidant activity, total polyphenol content, pH, and organoleptic properties. The addition of OPP significantly increased antioxidant activity and total polyphenol content and decreased pH, indicating acidic nature of OPP. Polyphenols from OPP effectively suppressed lipid oxidation. A 1-2% addition of OPP enhanced sensory properties. After the 28-day storage, the control samples received the lowest sensory score, due to the presence of a strong fishy odor, which was not present in samples with OPP. HPLC-MS/MS analysis revealed that quercetin is the most dominant compound in OPP. Overall, the results indicate that the addition of OPP in amounts of 1-2% can extend shelf life, without the deterioration of sensory properties.

Department of Animal Husbandry Sciences Faculty of Agriculture University of South Bohemia in České Budějovice Studentská 1668 370 05 České Budějovice Czech Republic

Department of Food Biotechnologies and Agricultural Products' Quality Faculty of Agriculture University of South Bohemia in České Budějovice Studentská 1668 370 05 České Budějovice Czech Republic

Food Research Institute Prague Radiová 1285 7 102 00 Praha 10 Hostivař Czech Republic

South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Institute of Aquaculture and Protection of Waters Faculty of Fisheries and Protection of Waters University of South Bohemia in České Budějovice Na Sádkách 1780 370 05 České Budějovice Czech Republic

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