The aim of this study was to evaluate if cytoplasmic transfer can improve fertilization and embryo quality of women with oocytes of low quality. During ICSI, 10-15% of the cytoplasm from a fresh or frozen young donor oocyte was added to the recipient oocyte. According to the embryo quality, we defined group A as patients in which the best embryo was evident after cytoplasmic transfer and group B as patients in which the best embryo was evident after a simple ICSI. We investigated in the period of 2002-2018, 125 in vitro fertilization cycles involving 1011 fertilized oocytes. Five hundred fifty-seven sibling oocytes were fertilized using ICSI only and 454 oocytes with cytoplasmic transfer. Fertilization rates of oocytes were 67.2% in the cytoplasmic transfer and 53.5% in the ICSI groups (P < 0.001). A reduction in fertilization rate was observed with increased women age in the ICSI but not in the cytoplasmic transfer groups. The best embryo quality was found after cytoplasmic transfer in 78 cycles (62.4%) and without cytoplasmic transfer in 40 cycles (32%, P < 0.001). No significant differences were detected between the age, hormonal levels, dose of stimulation drugs, number of transferred embryos, pregnancy rate and abortion rate between A and B groups. Cytoplasmic transfer improves fertilization rates and early embryo development in humans with low oocyte quality. All 28 children resulting from cytoplasmic transfer are healthy.

Department of Ecology and Environmental Sciences Faculty of Science Palacký University Olomouc 77900 Olomouc Czech Republic

Department of Medical Genetics University Hospital Olomouc and Faculty of Medicine and Dentistry Palacký University Olomouc Olomouc Czech Republic

Department of Paediatrics University Hospital Olomouc and Faculty of Medicine and Dentistry Palacký University Olomouc Olomouc Czech Republic

Fertimed Infertility Center 77200 Olomouc Czech Republic

Olomouc Czech Republic

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Igarashi H, Takahashi T, Nagase S. Oocyte aging underlies female reproductive aging: biological mechanisms and therapeutic strategies. Reprod Med Biol. 2015;14:159–169. doi: 10.1007/s12522-015-0209-5. PubMed DOI PMC

Tarín JJ, Pérez-Albalá S, Cano A. Consequences on offspring of abnormal function in aging gametes. Hum Reprod Update. 2000;6:532–549. doi: 10.1093/humupd/6.6.532. PubMed DOI

Meldrum DR, Casper RF, Diez-Juan A, et al. Aging and the environment affect gamete and embryo potential: can we intervene? PLoS Genet. 2010;6:e1001066. doi: 10.1371/journal.pgen.1001066. PubMed DOI

Tachibana M, Sparman M, Sritanaudomchai H. Mitochondrial gene replacement in primate offspring and embryonic stem cells. Nature. 2009;491:367–372. doi: 10.1038/nature08368. PubMed DOI PMC

Chappel S. The role of mitochondria from mature oocyte to viable blastocyst. Obstet Gynecol Int. 2013;183024:1–10. doi: 10.1155/2013/183024. PubMed DOI PMC

Goud AP, Goud PT, Van Oostveldt P, et al. Dynamic changes in microtubular cytoskeleton of human postmature oocytes revert after ooplasm transfer. Fertil Steril. 2004;81:323–331. doi: 10.1016/j.fertnstert.2003.06.033. PubMed DOI

Cohen J, Scott R, Alikani M, Schimmel T, Munné S, Levron J, Wu L, Brenner C, Warner C, Willadsen S. Ooplasmic transfer in mature human oocytes. Mol Hum Reprod. 1998;4:269–280. doi: 10.1093/molehr/4.3.269. PubMed DOI

Blerkom J. Mitochondrial function in the human oocyte and embryo and their role in developmental competence. Mitochondrion. 2011;11:797–813. doi: 10.1016/j.mito.2010.09.012. PubMed DOI

Muggleton-Harris A, Whithingham D, Wilson L. Cytoplasmic control of preimplantation development development in vitro in mouse. Nature. 1982;299:460–462. doi: 10.1038/299460a0. PubMed DOI

Cohen J, Scott R, Schimmel T, Levron J, Willadsen S. Birth of infant after transfer of anucleate donor oocyte cytoplasma into recipient eggs. Lancet. 1997;350:186–187. doi: 10.1016/S0140-6736(05)62353-7. PubMed DOI

Lanzendorf SE, Mayer JF, Toner J, Oehninger S, Saffan DS, Muasher S. Pregnancy following transfer of ooplasm from cryopreserved-thawed donor oocytes into recipient oocytes. Fertil Steril. 1999;71:575–577. doi: 10.1016/S0015-0282(98)00504-4. PubMed DOI

Dale B, Wilding M, Botta G, Rasile M, Marino M, di Matteo L, de Placido G, Izzo A. Pregnancy after cytoplasmic transfer in a couple suffering from idiopathic infertility. Hum Reprod. 2001;16:1469–1472. doi: 10.1093/humrep/16.7.1469. PubMed DOI

Goud AP, Goud PT, Van Oostveldt P, et al. Dynamic changes in microtubular cytoskeleton of human postmature oocytes revert after ooplasm transfer. Fertil Steril. 2004;81:323–331. doi: 10.1016/j.fertnstert.2003.06.033. PubMed DOI

Gardner DK, Lane M, Schoolcraft WB. Physiology and culture of the human blastocyst. J Reprod Immunol. 2002;55:85–100. doi: 10.1016/S0165-0378(01)00136-X. PubMed DOI

Sperandei S. Understanding logistic regression analysis. Biochem Medica. 2014;24:12–18. doi: 10.11613/BM.2014.003. PubMed DOI PMC

R Core Team. R: A language and environment for statistical computing. 2017 Vienna, Austria: R Foundation for Statistical Computing.

Darbandi S, Darbandi M, Khorshid HRK, et al. Ooplasmic transfer in human oocytes: efficacy and concerns in assisted reproduction. Reprod Biol Endocrinol. 2017;15:77. doi: 10.1186/s12958-017-0292-z. PubMed DOI PMC

Chen SH, Pascale C, Jackson M, Szvetecz MA, Cohen J. A limited survey-based uncontrolled follow-up study of children born after ooplasmic transplantation in a single Centre. Reprod BioMed Online. 2016;33:737–744. doi: 10.1016/j.rbmo.2016.10.003. PubMed DOI

Cohen J, Alikani M. The biological baisis for defining bi-parental or tri-parental origin of offspring from cytoplasmic and spindle transfer. Reprod BioMed Online. 2013;26:515–537. PubMed

Caicedo A, Aponte PM, Cabrera F, et al. Artificial mitochondria transfer: current challenges, advances, and future applications. Stem Cells Int. 2017;7610414. PubMed PMC

Barrit JA, Brenner CA, Malter HE, et al. Mitochodnria in human offspring derived from ooplasmic transplantation: brief communication. Hum Reprod. 2001;16:513–516. doi: 10.1093/humrep/16.3.513. PubMed DOI

Malter HE, Cohen J. Ooplasmic transfer: animal models assist human studies. Reprod BioMed Online. 2002;5:26–35. doi: 10.1016/S1472-6483(10)61593-3. PubMed DOI

Sobek AJ, Tkadlec E, Hladíková B, et al. Is there a decling trend in ovarian function among infertility clinic patients? Hum Reprod. 2010;25:127–132. doi: 10.1093/humrep/dep372. PubMed DOI

Cree L, Loid P. Mitochondrial replacement: from basic research to assisted reproductive technology portfolio tool – technicalities and possible risks. Mol Hum Reprod. 2015;21:3–10. doi: 10.1093/molehr/gau082. PubMed DOI

Woods DC, Khrapko K, Tilly JL. Influence of maternal aging on mitochondrial heterogeneity, inheritance, and function in oocytes and preimplantation embryos. Genes. 2018; 9: piiE265. PubMed PMC

Hiendleder S, Wolf E. The mitochondrial genome in embryo technologies. Reprod Dom Anim. 2003;38:290–304. doi: 10.1046/j.1439-0531.2003.00448.x. PubMed DOI

Brenner CA, Barritt JA, Wiladsen S, et al. Mitochondrial DNA heteroplasmy after human ooplasmic transplantation. Fertil Steril. 2000;74:573–578. doi: 10.1016/S0015-0282(00)00681-6. PubMed DOI

Matthew VC, Kumiko T, Carl AP. Mitochondrial biology in reproduction. Reprod Med Biol. 2011;10:251–258. doi: 10.1007/s12522-011-0101-x. PubMed DOI PMC

Burgstaller JP, Johnston IG, Poulton J. Mitochondrial DNA disease and developmental implications for reproductive strategies. Mol Hum Reprod. 2015;21:11–22. doi: 10.1093/molehr/gau090. PubMed DOI PMC

Hoseini FS, Salsabili N, Akbari-Asbagh F, Aflatoonian R, Aghaee-Bakhtiari SH. Comparison of gene expression profiles in human germinal vesicle before and after cytoplasmic transfer from mature oocytes in Iranian infertile couples. J Family Reprod Health. 2016;10:71–79. PubMed PMC

Ivanov PL, Wadhams MJ, Roby RK, et al. Mitochondrial DNA sequence heteroplasmy in the grand Duke of Russia Georgij Romanov establishes the authenticity of the remains of tsar Nicholas II. Nat Genet. 1996;4:417–420. doi: 10.1038/ng0496-417. PubMed DOI

Poulton J, Chiaratti MR, Meirelles FV, et al. Transmission of Mitochondrial DNA Diseases and Ways to Prevent Them. PLoS Genet. 2010: 6(8): e1001066. 10.1371/journal.pgen.1001066. PubMed PMC

Ferreira CR, Burgstaller JP, Perecin F, Garcia JM, Chiaratti MR, Méo SC, Müller M, Smith LC, Meirelles FV, Steinborn R. Pronounced segregation of donor mitochondria introduced by bovine ooplasmic transfer to the female germ-line. Biol Reprod. 2010;82:563–571. doi: 10.1095/biolreprod.109.080564. PubMed DOI

Diot A, Dombi E, Lodge T, Liao C, Morten K, Carver J, Wells D, Child T, Johnston IG, Williams S, Poulton J. Modulationg mitochondrial quality in disease transmiaaion: towards enabling mitochondrial DNA disease carriers to have healthy children. Biochem Soc Trans. 2016;44:1091–1100. doi: 10.1042/BST20160095. PubMed DOI PMC

Edwards R. Causes of early pregnancy loss. Hum Reprod. 1986;1:185–198. doi: 10.1093/oxfordjournals.humrep.a136378. PubMed DOI

An Interplay between Epigenetics and Translation in Oocyte Maturation and Embryo Development: Assisted Reproduction Perspective