Structural studies of challenging targets such as G protein-coupled receptors (GPCRs) have accelerated during the last several years due to the development of new approaches, including small-wedge and serial crystallography. Here, we describe the deposition of seven datasets consisting of X-ray diffraction images acquired from lipidic cubic phase (LCP) grown microcrystals of two human GPCRs, Cysteinyl leukotriene receptors 1 and 2 (CysLT1R and CysLT2R), in complex with various antagonists. Five datasets were collected using small-wedge synchrotron crystallography (SWSX) at the European Synchrotron Radiation Facility with multiple crystals under cryo-conditions. Two datasets were collected using X-ray free electron laser (XFEL) serial femtosecond crystallography (SFX) at the Linac Coherent Light Source, with microcrystals delivered at room temperature into the beam within LCP matrix by a viscous media microextrusion injector. All seven datasets have been deposited in the open-access databases Zenodo and CXIDB. Here, we describe sample preparation and annotate crystallization conditions for each partial and full datasets. We also document full processing pipelines and provide wrapper scripts for SWSX and SFX data processing.

Bridge Institute Michelson Center for Convergent Bioscience University of Southern California Los Angeles CA 90089 USA

Department of Chemistry University of Southern California Los Angeles CA 90089 USA

ELI Beamlines Institute of Physics Czech Academy of Science Na Slovance 2 18221 Prague Czech Republic

ESRF The European Synchrotron 38000 Grenoble France

Institut de Biologie Structurale Université Grenoble Alpes CEA CNRS Grenoble France

Institute of Biological Information Processing Forschungszentrum Jülich GmbH 52425 Jülich Germany

Institute of Crystallography University of Aachen Aachen Germany

Joint Institute for Nuclear Research Dubna 141980 Russia

JuStruct Jülich Center for Structural Biology Forschungszentrum Jülich GmbH 52425 Jülich Germany

MRC Laboratory of Molecular Biology Cambridge CB2 0QH UK

Research Сenter for Molecular Mechanisms of Aging and Age related Diseases Moscow Institute of Physics and Technology Dolgoprudny 141701 Russia

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Sci Data. 2020 Nov 23;7(1):419. doi: 10.1038/s41597-020-00759-w. PubMed

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