Single Particle Imaging (SPI) with intense coherent X-ray pulses from X-ray free-electron lasers (XFELs) has the potential to produce molecular structures without the need for crystallization or freezing. Here we present a dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source (LCLS). Additional exposures with background information are also deposited. The diffraction data were collected at the Atomic, Molecular and Optical Science Instrument (AMO) of the LCLS in 4 experimental beam times during a period of four years. The photon energy was either 1.2 or 1.7 keV and the pulse energy was between 2 and 4 mJ in a focal spot of about 1.3 μm x 1.7 μm full width at half maximum (FWHM). The X-ray laser pulses captured the particles in random orientations. The data offer insight into aerosolised virus particles in the gas phase, contain information relevant to improving experimental parameters, and provide a basis for developing algorithms for image analysis and reconstruction.

ARC Centre of Excellence in Advanced Molecular Imaging La Trobe University Bundoora VIC 3086 Australia

ARC Centre of Excellence in Advanced Molecular Imaging School of Physics University of Melbourne Parkville Victoria 3010 Australia

Arizona State University 1001S McAllister Avenue Tempe AZ 85287 USA

Brookhaven National Laboratory Bldg 535B Upton NY 11973 USA

Center for Free Electron Laser Science DESY Notkestrasse 85 22607 Hamburg Germany

Centre for Ultrafast Imaging Luruper Chaussee 149 22761 Hamburg Germany

Department of Applied Physics KTH Royal Institute of Technology AlbaNova University Center KTH Royal Institute of Technology S 106 91 Stockholm Sweden

Department of Chemistry and Physics La Trobe Institute for Molecular Science La Trobe University Melbourne Victoria 3086 Australia

Department of Physics National University of Singapore 14 Science Drive 4 Blk S1A Level 2 S1A 02 07 Lee Wee Kheng Building Singapore 117557 Singapore

DESY Photon Science Notkestrasse 85 22607 Hamburg Germany

Diamond Light Source Harwell Science and Innovation Campus Didcot OX11 0DE United Kingdom

European XFEL Holzkoppel 4 22869 Schenefeld Germany

Koc University Rumelifeneri Sariyer Rumeli Feneri Yolu 34450 Sariyer Istanbul Turkey

Laboratory of Molecular Biophysics Department of Cell and Molecular Biology Uppsala University Husargatan 3 SE 751 24 Uppsala Sweden

Lawrence Livermore National Laboratory 7000 East Avenue L 452 Livermore California 94550 USA

Max Planck Institute for the Structure and Dynamics of Matter Luruper Chaussee 149 22761 Hamburg Germany

NRNU MEPhI Kashirskoe shosse 31 115409 Moscow Russia

Photon Science Division Paul Scherrer Institute CH 5232 Villigen PSI Switzerland

Physics Department Stanford University 450 Serra Mall Stanford California 94305 USA

SLAC National Accelerator Laboratory 2575 Sand Hill Road Menlo Park California 94025 USA

Stanford PULSE Institute 2575 Sand Hill Road Menlo Park California 94025 USA

The European Extreme Light Infrastructure Institute of Physics Academy of Sciences of the Czech Republic Za Radnicic 835 25241 Dolní Břežany Czech Republic

University of Wisconsin Milwaukee 3135N Maryland Ave Milwaukee Wisconsin 53211 USA

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Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source