Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65-70 nm, which is considerably smaller than the previously reported ~600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.

Argonne National Laboratory 9700 S Cass Ave Lemont Illinois 60439 USA

Arizona State University School of Life Sciences Tempe Arizona 85287 5401 USA

Australian Research Council Centre of Excellence in Advanced Molecular Imaging La Trobe Institute for Molecular Science La Trobe University Melbourne 3086 Australia

Biodesign Center for Applied Structural Discovery Biodesign Institute at Arizona State University Tempe Arizona 85287 5001 USA

Biodesign Center for Immunotherapy Vaccines and Virotherapy Biodesign Institute at Arizona State University Tempe Arizona 85287 USA

Biomedical and 10 Ray Physics Department of Applied Physics AlbaNova University Center KTH Royal Institute of Technology Stockholm SE 106 91 Sweden

Center for Free Electron Laser Science DESY Notkestrasse 85 22607 Hamburg Germany

Centre for Bio imaging Sciences National University of Singapore Blk S1A 14 Science Drive 4 Singapore 117557

Centre for Ultrafast Imaging University of Hamburg Luruper Chaussee 149 22761 Hamburg Germany

Department of Physics Arizona State University Tempe Arizona 85287 USA

Department of Physics National University of Singapore Blk S12 2 Science Drive 3 Singapore 117551

Department of Physics Pohang University of Science and Technology Pohang 37673 Korea

Department of Physics University of Hamburg Luruper Chausee 149 22761 Hamburg Germany

Department of Physics University of Wisconsin Milwaukee KIRC 3135 N Maryland Ave Milwaukee Wisconsin 53211 USA

Deutsches Elektronen Synchrotron DESY Notkestraße 85 D 22607 Hamburg Germany

Division of Scientific Computing Department of Information Technology Science for Life Laboratory Uppsala University Husargatan 3 Uppsala SE 75105 Sweden

European 10 ray Free Electron Laser GmbH Holzkoppel 4 22869 Schenefeld Germany

Institut für Optik und Atomare Physik Technische Universität Berlin Hardenbergstraße 36 10623 Berlin Germany

Institute of Physics AS CR v v i Na Slovance 2 18221 Prague 8 Czech Republic

Laboratory of Molecular Biophysics Department of Cell and Molecular Biology Uppsala University Husargatan 3 Uppsala SE 75124 Sweden

Max Planck Institute for the Structure and Dynamics of Matter 22607 Hamburg Germany

National Research Centre 'Kurchatov Institute' Akademika Kurchatova pl 1 123182 Moscow Russia

National Research Nuclear University MEPhI Kashirskoe shosse 31 115409 Moscow Russia

NSLS 2 Brookhaven National Laboratory Upton New York 11873 USA

School of Molecular Sciences Arizona State University Tempe Arizona 85287 1604 USA

SLAC National Accelerator Laboratory 2575 Sand Hill Road Menlo Park California 94025 USA

Stanford PULSE Institute SLAC National Accelerator Laboratory 2575 Sand Hill Road Menlo Park California 94025 USA

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Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source

An advanced workflow for single-particle imaging with the limited data at an X-ray free-electron laser

Electron cryo-microscopy of bacteriophage PR772 reveals the elusive vertex complex and the capsid architecture

Rayleigh-scattering microscopy for tracking and sizing nanoparticles in focused aerosol beams