Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, was aerosolized and injected into the approximately 0.1 μm diameter focused hard X-ray beam at the CXI instrument of LCLS. Diffraction patterns from RDV with signal to 5.9 Ångström were recorded. The diffraction data are available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development, the contents of which are described here.

Argonne National Laboratory 9700 South Cass Avenue Argonne Illinois 60439 USA

Arizona State University Department of Physics Tempe Arizona 85287 USA

Arizona State University School of Life Sciences Tempe Arizona 85287 5401 USA

Arizona State University School of Molecular Sciences Tempe Arizona 85287 1604 USA

Australian Research Council Centre of Excellence in Advanced Molecular Imaging La Trobe Institute for Molecular Science La Trobe University Melbourne 3086 Australia

Beijing Computational Science Research Center 8 W Dongbeiwang Rd Haidian Beijing 100193 China

Biodesign Center for Applied Structural Discovery Biodesign Institute at Arizona State University Tempe 85287 USA

Biodesign Center for Infectious Diseases and Vaccinology Biodesign Institute at Arizona State University Tempe 85287 USA

Biomedical and 10 Ray Physics Department of Applied Physics AlbaNova University Center KTH Royal Institute of Technology Stockholm SE 106 91 Sweden

Brookhaven National Laboratory NSLS 2 Upton New York 11973 USA

Center for Free Electron Laser Science Deutsches Elektronen Synchrotron DESY Hamburg 22607 Germany

Centre for Bio imaging Sciences National University of Singapore 14 Science Drive 4 BLK S1A Singapore 117543 Singapore

Department of Information Technology Science for Life Laboratory Uppsala University Lägerhyddsvägen 2 Uppsala SE 75105 Sweden

Department of Physics and Astronomy Uppsala University Lägerhyddsvägen 1 Uppsala SE 75120 Sweden

Department of Physics Pohang University of Science and Technology Pohang 37673 Korea

Department of Physics University of Hamburg Hamburg 22761 Germany

Department of Physics University of Wisconsin Milwaukee 3135 North Maryland Ave Milwaukee Wisconsin 53211 USA

Deutsches Elektronen Synchrotron DESY Notkestraße 85 Hamburg D 22607 Germany

European XFEL GmbH Holzkoppel 4 Schenefeld 22869 Germany

Institut für Optik und Atomare Physik Technische Universität Berlin Hardenbergstraße 36 Berlin 10623 Germany

Institute for Protein Research Osaka University Suita Osaka 565 0871 Japan

Institute of Physics ASCR v v i ELI Beamlines Project Prague 182 21 Czech Republic

Laboratory of Atomic and Solid State Physics Cornell University Ithaca New York 14853 USA

Laboratory of Molecular Biophysics Department of Cell and Molecular Biology Uppsala University Husargatan 3 Uppsala SE 75124 Sweden

Max Planck Institute for the Structure and Dynamics of Matter CFEL Hamburg 22607 Germany

National Research Nuclear University MEPhI Kashirskoe shosse 31 Moscow 115409 Russia

School of Materials Science and Engineering Gwangju Institute of Science and Technology Gwangju 61005 Korea

SLAC National Accelerator Laboratory 2575 Sand Hill Road Menlo Park California 94025 USA

Stanford PULSE Institute 2575 Sand Hill Road Menlo Park California 94025 USA

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