Bacteriophage PR772, a member of the Tectiviridae family, has a 70 nm diameter icosahedral protein capsid that encapsulates a lipid membrane, dsDNA, and various internal proteins. An icosahedrally averaged CryoEM reconstruction of the wild-type virion and a localized reconstruction of the vertex region reveal the composition and the structure of the vertex complex along with new protein conformations that play a vital role in maintaining the capsid architecture of the virion. The overall resolution of the virion is 2.75 Å, while the resolution of the protein capsid is 2.3 Å. The conventional penta-symmetron formed by the capsomeres is replaced by a large vertex complex in the pseudo T = 25 capsid. All the vertices contain the host-recognition protein, P5; two of these vertices show the presence of the receptor-binding protein, P2. The 3D structure of the vertex complex shows interactions with the viral membrane, indicating a possible mechanism for viral infection.

Department of Applied Physics Biomedical and 10 Ray Physics KTH Royal Institute of Technology Stockholm Sweden

Department of Biochemistry and Biophysics Stockholm University Stockholm Sweden

Department of Cell and Molecular Biology Laboratory of Molecular Biophysics Uppsala University Uppsala Sweden

Institute of Physics ELI Beamlines Academy of Sciences of the Czech Republic Prague Czech Republic

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An advanced workflow for single-particle imaging with the limited data at an X-ray free-electron laser

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PDB
6Q5U