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Unveiling the DHX15-G-patch interplay in retroviral RNA packaging
A. Dostálková, I. Křížová, P. Junková, J. Racková, M. Kapisheva, R. Novotný, M. Danda, K. Zvonařová, L. Šinkovec, K. Večerková, L. Bednářová, T. Ruml, M. Rumlová
Language English Country United States
Document type Journal Article
Grant support
LX22NPO5103
Ministerstvo Školství, Mládeže a Tělovýchovy (MŠMT)
90254
Ministerstvo Školství, Mládeže a Tělovýchovy (MŠMT)
NLK
Free Medical Journals
from 1915 to 6 months ago
Freely Accessible Science Journals
from 1915 to 6 months ago
PubMed Central
from 1915 to 6 months ago
Europe PubMed Central
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Open Access Digital Library
from 1915-01-15
Open Access Digital Library
from 1915-01-01
- MeSH
- Cell Nucleus metabolism virology MeSH
- DEAD-box RNA Helicases metabolism genetics MeSH
- Genome, Viral MeSH
- HEK293 Cells MeSH
- Humans MeSH
- Mason-Pfizer monkey virus * genetics metabolism physiology MeSH
- Virus Replication genetics physiology MeSH
- RNA, Viral * metabolism genetics MeSH
- RNA Helicases metabolism genetics MeSH
- Virus Assembly * genetics physiology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
We explored how a simple retrovirus, Mason-Pfizer monkey virus (M-PMV) to facilitate its replication process, utilizes DHX15, a cellular RNA helicase, typically engaged in RNA processing. Through advanced genetic engineering techniques, we showed that M-PMV recruits DHX15 by mimicking cellular mechanisms, relocating it from the nucleus to the cytoplasm to aid in viral assembly. This interaction is essential for the correct packaging of the viral genome and critical for its infectivity. Our findings offer unique insights into the mechanisms of viral manipulation of host cellular processes, highlighting a sophisticated strategy that viruses employ to leverage cellular machinery for their replication. This study adds valuable knowledge to the understanding of viral-host interactions but also suggests a common evolutionary history between cellular processes and viral mechanisms. This finding opens a unique perspective on the export mechanism of intron-retaining mRNAs in the packaging of viral genetic information and potentially develop ways to stop it.
Department of Biotechnology University of Chemistry and Technology 166 28 Prague Czech Republic
Institute of Molecular Genetics Czech Academy of Sciences 142 20 Prague Czech Republic
References provided by Crossref.org
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