-
Je něco špatně v tomto záznamu ?
Unveiling the DHX15-G-patch interplay in retroviral RNA packaging
A. Dostálková, I. Křížová, P. Junková, J. Racková, M. Kapisheva, R. Novotný, M. Danda, K. Zvonařová, L. Šinkovec, K. Večerková, L. Bednářová, T. Ruml, M. Rumlová
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Grantová podpora
LX22NPO5103
Ministerstvo Školství, Mládeže a Tělovýchovy (MŠMT)
90254
Ministerstvo Školství, Mládeže a Tělovýchovy (MŠMT)
NLK
Free Medical Journals
od 1915 do Před 6 měsíci
Freely Accessible Science Journals
od 1915 do Před 6 měsíci
PubMed Central
od 1915 do Před 6 měsíci
Europe PubMed Central
od 1915 do Před 6 měsíci
Open Access Digital Library
od 1915-01-15
Open Access Digital Library
od 1915-01-01
PubMed
39320912
DOI
10.1073/pnas.2407990121
Knihovny.cz E-zdroje
- MeSH
- buněčné jádro metabolismus virologie MeSH
- DEAD-box RNA-helikasy metabolismus genetika MeSH
- genom virový MeSH
- HEK293 buňky MeSH
- lidé MeSH
- Masonův-Pfizerův opičí virus * genetika metabolismus fyziologie MeSH
- replikace viru genetika fyziologie MeSH
- RNA virová * metabolismus genetika MeSH
- RNA-helikasy metabolismus genetika MeSH
- sestavení viru * genetika fyziologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
We explored how a simple retrovirus, Mason-Pfizer monkey virus (M-PMV) to facilitate its replication process, utilizes DHX15, a cellular RNA helicase, typically engaged in RNA processing. Through advanced genetic engineering techniques, we showed that M-PMV recruits DHX15 by mimicking cellular mechanisms, relocating it from the nucleus to the cytoplasm to aid in viral assembly. This interaction is essential for the correct packaging of the viral genome and critical for its infectivity. Our findings offer unique insights into the mechanisms of viral manipulation of host cellular processes, highlighting a sophisticated strategy that viruses employ to leverage cellular machinery for their replication. This study adds valuable knowledge to the understanding of viral-host interactions but also suggests a common evolutionary history between cellular processes and viral mechanisms. This finding opens a unique perspective on the export mechanism of intron-retaining mRNAs in the packaging of viral genetic information and potentially develop ways to stop it.
Department of Biotechnology University of Chemistry and Technology 166 28 Prague Czech Republic
Institute of Molecular Genetics Czech Academy of Sciences 142 20 Prague Czech Republic
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc24018729
- 003
- CZ-PrNML
- 005
- 20241024111239.0
- 007
- ta
- 008
- 241015s2024 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1073/pnas.2407990121 $2 doi
- 035 __
- $a (PubMed)39320912
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Dostálková, Alžběta $u Department of Biotechnology, University of Chemistry and Technology, 166 28 Prague, Czech Republic
- 245 10
- $a Unveiling the DHX15-G-patch interplay in retroviral RNA packaging / $c A. Dostálková, I. Křížová, P. Junková, J. Racková, M. Kapisheva, R. Novotný, M. Danda, K. Zvonařová, L. Šinkovec, K. Večerková, L. Bednářová, T. Ruml, M. Rumlová
- 520 9_
- $a We explored how a simple retrovirus, Mason-Pfizer monkey virus (M-PMV) to facilitate its replication process, utilizes DHX15, a cellular RNA helicase, typically engaged in RNA processing. Through advanced genetic engineering techniques, we showed that M-PMV recruits DHX15 by mimicking cellular mechanisms, relocating it from the nucleus to the cytoplasm to aid in viral assembly. This interaction is essential for the correct packaging of the viral genome and critical for its infectivity. Our findings offer unique insights into the mechanisms of viral manipulation of host cellular processes, highlighting a sophisticated strategy that viruses employ to leverage cellular machinery for their replication. This study adds valuable knowledge to the understanding of viral-host interactions but also suggests a common evolutionary history between cellular processes and viral mechanisms. This finding opens a unique perspective on the export mechanism of intron-retaining mRNAs in the packaging of viral genetic information and potentially develop ways to stop it.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a buněčné jádro $x metabolismus $x virologie $7 D002467
- 650 _2
- $a DEAD-box RNA-helikasy $x metabolismus $x genetika $7 D053487
- 650 _2
- $a genom virový $7 D016679
- 650 _2
- $a HEK293 buňky $7 D057809
- 650 12
- $a Masonův-Pfizerův opičí virus $x genetika $x metabolismus $x fyziologie $7 D016093
- 650 _2
- $a RNA-helikasy $x metabolismus $x genetika $7 D020365
- 650 12
- $a RNA virová $x metabolismus $x genetika $7 D012367
- 650 12
- $a sestavení viru $x genetika $x fyziologie $7 D019065
- 650 _2
- $a replikace viru $x genetika $x fyziologie $7 D014779
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Křížová, Ivana $u Department of Biotechnology, University of Chemistry and Technology, 166 28 Prague, Czech Republic $1 https://orcid.org/0000000204974924
- 700 1_
- $a Junková, Petra $u Institute of Organic Chemistry and Biochemistry Research Centre & Gilead Sciences, Czech Academy of Sciences, 166 10 Prague, Czech Republic $u Department of Biochemistry and Microbiology, University of Chemistry and Technology 166 28, Prague, Czech Republic
- 700 1_
- $a Racková, Jana $u Department of Biotechnology, University of Chemistry and Technology, 166 28 Prague, Czech Republic $1 https://orcid.org/0000000332624937
- 700 1_
- $a Kapisheva, Marina $u Department of Biotechnology, University of Chemistry and Technology, 166 28 Prague, Czech Republic $1 https://orcid.org/0000000262459592
- 700 1_
- $a Novotný, Radim $u Department of Biochemistry and Microbiology, University of Chemistry and Technology 166 28, Prague, Czech Republic
- 700 1_
- $a Danda, Matěj $u Department of Biotechnology, University of Chemistry and Technology, 166 28 Prague, Czech Republic $1 https://orcid.org/000000020828412X
- 700 1_
- $a Zvonařová, Karolína $u Department of Biotechnology, University of Chemistry and Technology, 166 28 Prague, Czech Republic $1 https://orcid.org/0000000174928379
- 700 1_
- $a Šinkovec, Larisa $u Department of Biotechnology, University of Chemistry and Technology, 166 28 Prague, Czech Republic $1 https://orcid.org/0000000263153931
- 700 1_
- $a Večerková, Kateřina $u Department of Informatics and Chemistry, University of Chemistry and Technology, 166 28 Prague, Czech Republic $u Institute of Molecular Genetics, Czech Academy of Sciences, 142 20 Prague, Czech Republic $1 https://orcid.org/0009000773878868
- 700 1_
- $a Bednářová, Lucie $u Institute of Organic Chemistry and Biochemistry Research Centre & Gilead Sciences, Czech Academy of Sciences, 166 10 Prague, Czech Republic
- 700 1_
- $a Ruml, Tomáš $u Department of Biochemistry and Microbiology, University of Chemistry and Technology 166 28, Prague, Czech Republic $1 https://orcid.org/0000000256984366 $7 nlk20030128618
- 700 1_
- $a Rumlová, Michaela $u Department of Biotechnology, University of Chemistry and Technology, 166 28 Prague, Czech Republic $1 https://orcid.org/0000000256458227 $7 ola20040226003
- 773 0_
- $w MED00010472 $t Proceedings of the National Academy of Sciences of the United States of America $x 1091-6490 $g Roč. 121, č. 40 (2024), s. e2407990121
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/39320912 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y - $z 0
- 990 __
- $a 20241015 $b ABA008
- 991 __
- $a 20241024111233 $b ABA008
- 999 __
- $a ok $b bmc $g 2201539 $s 1230702
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2024 $b 121 $c 40 $d e2407990121 $e 20240925 $i 1091-6490 $m Proceedings of the National Academy of Sciences of the United States of America $n Proc Natl Acad Sci U S A $x MED00010472
- GRA __
- $a LX22NPO5103 $p Ministerstvo Školství, Mládeže a Tělovýchovy (MŠMT)
- GRA __
- $a 90254 $p Ministerstvo Školství, Mládeže a Tělovýchovy (MŠMT)
- LZP __
- $a Pubmed-20241015
