Gold nanoparticles coated with polyvinylpyrrolidone and sea urchin extracellular molecules induce transient immune activation
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
33254802
DOI
10.1016/j.jhazmat.2020.123793
PII: S0304-3894(20)31782-9
Knihovny.cz E-zdroje
- Klíčová slova
- Immune metabolic rewiring, Immunoreactivity, Innate defence response, Nano-recognition, Sea urchin immune cells,
- MeSH
- fagocyty MeSH
- kovové nanočástice * toxicita MeSH
- lidé MeSH
- Paracentrotus * MeSH
- povidon MeSH
- zlato MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- povidon MeSH
- zlato MeSH
We report that the immunogenicity of colloidal gold nanoparticles coated with polyvinylpyrrolidone (PVP-AuNPs) in a model organism, the sea urchin Paracentrotus lividus, can function as a proxy for humans for in vitro immunological studies. To profile the immune recognition and interaction from exposure to PVP-AuNPs (1 and 10 μg mL-1), we applied an extensive nano-scale approach, including particle physicochemical characterisation involving immunology, cellular biology, and metabolomics. The interaction between PVP-AuNPs and soluble proteins of the sea urchin physiological coelomic fluid (blood equivalent) results in the formation of a protein "corona" surrounding the NPs from three major proteins that influence the hydrodynamic size and colloidal stability of the particle. At the lower concentration of PVP-AuNPs, the P. lividus phagocytes show a broad metabolic plasticity based on the biosynthesis of metabolites mediating inflammation and phagocytosis. At the higher concentration of PVP-AuNPs, phagocytes activate an immunological response involving Toll-like receptor 4 (TLR4) signalling pathway at 24 hours of exposure. These results emphasise that exposure to PVP-AuNPs drives inflammatory signalling by the phagocytes and the resolution at both the low and high concentrations of the PVP-AuNPs and provides more details regarding the immunogenicity of these NPs.
Catalan Institute of Nanoscience and Nanotechnology Barcelona Spain
Consiglio Nazionale delle Ricerche Istituto per la Ricerca e l'Innovazione Biomedica Palermo Italy
Institute of Microbiology of the Czech Academy of Sciences Prague Czech Republic
SYSBIO IT Centre of Systems Biology University of Milano Bicocca Milano Italy
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