Extracellular vesicles secreted by model tapeworm Hymenolepis diminuta: biogenesis, ultrastructure and protein composition
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
33307002
DOI
10.1016/j.ijpara.2020.09.010
PII: S0020-7519(20)30317-9
Knihovny.cz E-resources
- Keywords
- Electron microscopy, Exosomes, LC–MS/MS, Proteins, Secretion, Size exclusion chromatography,
- MeSH
- Cestode Infections * MeSH
- Extracellular Vesicles * MeSH
- Hymenolepis diminuta * MeSH
- Hymenolepis * MeSH
- Host-Parasite Interactions MeSH
- Proteomics MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
We provided the first known evidence of the presence and release of extracellular vesicles in adults of important model tapeworm Hymenolepis diminuta. Two different subtypes have been observed on the surface of the worm and among the secretory products confirmed by several microscopical methods. Proteomic analysis revealed the presence of parasite-specific proteins as well as those of the host in purified extracellular vesicles. Among the protein cargo, we identified potential drug targets, vaccine candidates and H. diminuta antigens. Finally, the protein composition further revealed proteins participating in the endosomal complex required for transport-dependent biogenesis pathway.
References provided by Crossref.org
Secretion of extracellular vesicles during ontogeny of the tapeworm Schistocephalus solidus
