Extracellular vesicles secreted by model tapeworm Hymenolepis diminuta: biogenesis, ultrastructure and protein composition
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
33307002
DOI
10.1016/j.ijpara.2020.09.010
PII: S0020-7519(20)30317-9
Knihovny.cz E-zdroje
- Klíčová slova
- Electron microscopy, Exosomes, LC–MS/MS, Proteins, Secretion, Size exclusion chromatography,
- MeSH
- cestodózy * MeSH
- extracelulární vezikuly * MeSH
- Hymenolepis diminuta * MeSH
- Hymenolepis * MeSH
- interakce hostitele a parazita MeSH
- proteomika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
We provided the first known evidence of the presence and release of extracellular vesicles in adults of important model tapeworm Hymenolepis diminuta. Two different subtypes have been observed on the surface of the worm and among the secretory products confirmed by several microscopical methods. Proteomic analysis revealed the presence of parasite-specific proteins as well as those of the host in purified extracellular vesicles. Among the protein cargo, we identified potential drug targets, vaccine candidates and H. diminuta antigens. Finally, the protein composition further revealed proteins participating in the endosomal complex required for transport-dependent biogenesis pathway.
Citace poskytuje Crossref.org
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