Bioactive Steroids from the Red Sea Soft Coral Sinularia polydactyla
Jazyk angličtina Země Švýcarsko Médium electronic
Typ dokumentu srovnávací studie, časopisecké články
Grantová podpora
70/3/14684
National and Kapodistrian University of Athens
70/3/14685
National and Kapodistrian University of Athens
19-01383S
Czech Science Foundation
20-15621S
Czech Science Foundation
IGA_PrF_2020_021
Czech Science Foundation
PubMed
33322046
PubMed Central
PMC7763444
DOI
10.3390/md18120632
PII: md18120632
Knihovny.cz E-zdroje
- Klíčová slova
- Sinularia polydactyla, androgen receptor, anti-inflammatory, cytotoxic, neuroprotective, soft coral, steroids,
- MeSH
- antiflogistika izolace a purifikace farmakologie MeSH
- antitumorózní látky izolace a purifikace farmakologie MeSH
- endoteliální buňky pupečníkové žíly (lidské) účinky léků metabolismus MeSH
- fyziologická neovaskularizace účinky léků MeSH
- HeLa buňky MeSH
- inhibitory angiogeneze izolace a purifikace farmakologie MeSH
- korálnatci chemie MeSH
- lidé MeSH
- MFC-7 buňky MeSH
- molekulární struktura MeSH
- nádory farmakoterapie metabolismus patologie MeSH
- neurony účinky léků metabolismus patologie MeSH
- neuroprotektivní látky izolace a purifikace farmakologie MeSH
- steroidy izolace a purifikace farmakologie MeSH
- viabilita buněk účinky léků MeSH
- vztahy mezi strukturou a aktivitou MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Geografické názvy
- Indický oceán MeSH
- Názvy látek
- antiflogistika MeSH
- antitumorózní látky MeSH
- inhibitory angiogeneze MeSH
- neuroprotektivní látky MeSH
- steroidy MeSH
Six new (1, 2, 6, 8, 13, and 20) and twenty previously isolated (3-5, 7, 9-12, 14-19, and 21-26) steroids featuring thirteen different carbocycle motifs were isolated from the organic extract of the soft coral Sinularia polydactyla collected from the Hurghada reef in the Red Sea. The structures and the relative configurations of the isolated natural products have been determined based on extensive analysis of their NMR and MS data. The cytotoxic, anti-inflammatory, anti-angiogenic, and neuroprotective activity of compounds 3-7, 9-12, 14-20, and 22-26, as well as their effect on androgen receptor-regulated transcription was evaluated in vitro in human tumor and non-cancerous cells. Steroids 22 and 23 showed significant cytotoxicity in the low micromolar range against the HeLa and MCF7 cancer cell lines, while migration of endothelial cells was inhibited by compounds 11, 12, 22, and 23 at 20 µM. The results of the androgen receptor (AR) reporter assay showed that compound 11 exhibited the strongest inhibition of AR at 10 µM, while it is noteworthy that steroids 10, 16, and 20 displayed increased inhibition of AR with decreasing concentrations. Additionally, compounds 11 and 23 showed neuroprotective activity on neuron-like SH-SY5Y cells.
Department of Biochemistry Faculty of Agriculture Fayoum University Fayoum 63514 Egypt
Department of Zoology Faculty of Science Al Azhar University 71524 Assiut Egypt
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