It is commonly accepted that crime scene recovery and recording are key moments of any judicial inspection in which investigators must decide on the correct strategies to put into place. Complex outdoor scenarios, presenting partially or entirely skeletonised remains, can benefit more than others by the intervention of environmental specialists (forensic anthropologists, archaeologists, entomologists and botanists). These experts are capable of singling out, correctly recording and recovering environmental evidence that can lead to a more comprehensive reconstruction of a given criminal episode. If human remains are discovered in an outdoor scenario, the on-site presence of a botanist will guarantee a correct approach to the identification, recording and recovery of any botanical evidence. If an on-site botanist is not available, the operators must be capable of both the botanical evaluation of a scene and the implementation of correct botanical sampling protocols.The following collection of unusual case histories that aim at underlining the efficacy of forensic botany will examine the determination of post mortem or the post depositional interval, evidence for a victim's post mortem transfer, evidence for the identification of a primary crime scene and evidence for the identification of a victim's dismemberment site. In another two cases, one, we will illustrate the important role that forensic botany played in the discrimination between botanical material used to voluntarily conceal a victim and vegetation that had grown naturally above a disposal site, whereas the other will highlight the protocols implemented for the identification of a murder weapon.

Department of Anthropology Masaryk University Brno Czech Republic

Dipartimento di Bioscienze Università degli Studi di Milano Milan Italy

Labanof Sezione di Medicina Legale Dipartimento di Scienze Biomediche per la Salute Università degli Studi di Milano Milan Italy

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Bajerlein D, Wojterska M. Grewling Ł, Kokociński M. Forensic botany: current state of knowledge and possible applications in investigative practice. Issues of Forensics. Science. 2015;283:72–83.

Coyle MH. Forensic botany: principles and applications to criminal casework. Boca Raton: CRC Press; 2005.

Margiotta G, Bacaro G, Carnevali E, Severini S, Bacci M, Gabrielli M. Forensic botany as useful tool in the crime scene: report of a case. J Forensic Legal Med. 2015;34:24–28. doi: 10.1016/j.jflm.2015.05.003. PubMed DOI

Coyle MH, Lee CL, Lin WY, Lee HC, Palmbach TM. Forensic botany: using plant evidence to aid in forensic death investigation. Croat Med J. 2005;46(4):606–612. PubMed

Aquila I, Ausania F, Di Nunzio C, Serra A, Boca S, Capelli A, Magni P, Ricci P (2014) The role of forensic botany in crime scene investigation: case report and review of literature. J Forensic Sci 59(3):820–824. 10.1111/1556-4029.12401 PubMed

Aquila I, Sacco M, Ricci P, Gratteri S. The role of forensic botany in reconstructing the dynamics of trauma from high falls. J Forensic Sci. 2018;64(3):920–924. doi: 10.1111/1556-4029.13934. PubMed DOI

Lane MA, Anderson LC, Barkley TM, Bock JH, Gifford EM, Hall DW, Norris DO, Rost TL, Stern WL. Forensic botany: Plants, perpetrators, pests, poisons, and pot. BioScience. 1990;40(1):34–39. doi: 10.2307/1311237. DOI

Quatrehomme G, Lacoste A, Bailet P, Grevin G, Ollier A. Contribution of microscopic plant anatomy to post-mortem bone dating. J Forensic Sci. 1997;42(1):140–143. doi: 10.1520/JFS14084J. PubMed DOI

Brown A, Smith A, Elmhurst O. The combined use of pollen and soil analyses in a search and subsequent murder investigation. J Forensic Sci. 2002;47(3):614–618. doi: 10.1520/JFS15302J. PubMed DOI

Brown AG. The use of forensic botany and geology in war crimes investigations in NE Bosnia. Forensic Sci Int. 2006;163(3):204–210. doi: 10.1016/j.forsciint.2006.05.025. PubMed DOI

Montali E, Mercuri AM, Trevisan Grandi G, Accorsi CA. Towards a "crime pollen calendar" - pollen analysis on corpses throughout one year. Forensic Sci Int. 2006;163(3):211–223. doi: 10.1016/j.forsciint.2005.11.020. PubMed DOI

Caccianiga M, Bottacin S, Cattaneo C. Vegetation dynamics as a tool for detecting clandestine graves. J Forensic Sci. 2012;57(4):983–988. doi: 10.1111/j.1556-4029.2012.02071.x. PubMed DOI

Mildenhall DC. An unusual appearance of a common pollen type indicates the scene of the crime. Forensic Sci Int. 2006;163(3):236–240. doi: 10.1016/j.forsciint.2005.11.029. PubMed DOI

Mildenhall DC. Hypericum pollen determines the presence of burglars at the scene of a crime: an example of forensic palynology. Forensic Sci Int. 2006;163(3):231–235. doi: 10.1016/j.forsciint.2005.11.028. PubMed DOI

Wiltshire PE, Hawksworth DL, Webb JA, Edwards KJ. Two sources and two kinds of trace evidence: enhancing the links between clothing, footwear and crime scene. Forensic Sci Int. 2015;254:231–242. doi: 10.1016/j.forsciint.2015.05.033. PubMed DOI

Aquila I, Gratteri S, Sacco M, Ricci P. The role of forensic botany in solving a case: scientific evidence on the falsification of a crime scene. J Forensic Sci. 2018;63(3):961–964. doi: 10.1111/1556-4029.13639. PubMed DOI

Zaya DN, Ashley MV. Plant genetics for forensic applications. Methods Mol Biol. 2012;862:35–52. doi: 10.1007/978-1-61779-609-8_4. PubMed DOI

Wiltshire PE. Hair as a source of forensic evidence in murder investigations. Forensic Sci Int. 2006;163(3):241–248. doi: 10.1016/j.forsciint.2006.06.070. PubMed DOI

Willey P, Heilman A. Estimating time since death using plant roots and stems. J Forensic Sci. 1987;32(5):1264–1270. doi: 10.1520/JFS11177J. DOI

Courtin GM, Fairgrieve SL. Estimation of postmortem interval (PMI) as revealed through the analysis of annual growth in woody tissue. J Forensic Sci. 2004;49(4):1–3. doi: 10.1520/JFS2003419. PubMed DOI

Lancia M, Conforti F, Aleffi M, Caccianiga M, Bacci M, Rossi R. The use of Leptodyctium riparium (Hedw.) Warnst in the estimation of minimum postmortem interval. J Forensic Sci. 2012;58(S1):S239–S242. doi: 10.1111/1556-4029.12024. PubMed DOI

Caccianiga M, Compostella C (2009) Botanica e zoologia forense. In: Intini A, Picozzi M (eds) Scienze forensi: teoria e prassi dell’investigazione scientifica. UTET, Torino, pp 230–257

Cardoso HF, Santos A, Dias R, Garcia C, Pinto M, Sergio C. Establishing a minimum postmortem interval of human remains in an advanced state of skeletonization using the growth rate of bryophytes and plant roots. Int J Legal Med. 2010;124:451–456. doi: 10.1007/s00414-009-0372-5. PubMed DOI

Caccianiga M, Conforti F, Lancia M. Forensic botany: the use of mosses in criminal investigations. In: Mohamed J, editor. Moss: classification, development and growth and functional role in ecosystem. Nova. New York: Science Publisher; 2014. pp. 221–232.

Coyle MH, Ladd C, Palmbach T, Lee HC. The Green Revolution: botanical contributions to forensic and drug enforcement. Croat Med J. 2001;42(3):340–345. PubMed