Injectable materials have shown great potential in tissue engineering applications. However, bacterial infection is one of the main challenges in using these materials in the field of regenerative medicine. In this study, biogenically synthesized silver nanoparticle-decorated multi-walled carbon nanotubes (Ag/MWCNTs) were deployed for adorning biogenic-derived AgNPs which were subsequently used in the preparation of thermosensitive hydrogels based on hyaluronic acid encompassing these green-synthesized NPs. The antibacterial capacity of AgNPs decorated on MWCNTs synthesized through Camellia sinensis extract in an organic solvent-free medium displayed a superior activity by inhibiting the growth of Gram-negative (E. coli and Klebsiella) and Gram-positive (S. aureus and E. faecalis). The injectable hydrogel nanocomposites demonstrated good mechanical properties, as well. The thermosensitive hyaluronic acid-based hydrogels also exhibited Tgel below the body temperature, indicating the transition from liquid-like behavior to elastic gel-like behavior. Such a promising injectable nanocomposite could be applied as liquid, pomade, or ointment to enter wound cavities or bone defects and subsequently its transition in situ to gel form at human body temperature bodes well for their immense potential application in the biomedical sector.

Chemistry Department Faculty of Science Shahid Chamran University of Ahvaz 61537 53843 Ahvaz Iran

Department of Basic Science Faculty of Veterinary Medicine University of Tabriz 51666 16471 Tabriz Iran

Department of Biotechnology Faculty of Biological Science and Technology University of Isfahan Isfahan Iran

Istituto Italiano di Tecnologia Centre for Materials Interface viale Rinaldo Piaggio 34 56025 Pontedera Pisa Italy

Medical Technology Research Center Institute of Health Technology Kermanshah University of Medical Sciences 6715847141 Kermanshah Iran

Radiology and Nuclear Medicine Department School of Paramedical Sciences Kermanshah University of Medical Sciences Kermanshah Iran

Regional Centre of Advanced Technologies and Materials Palacky University Slechtitelu 27 783 71 Olomouc Czech Republic

Sabanci University Nanotechnology Research and Application Center 34956 Tuzla Istanbul Turkey

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