Xanthomonas campestris pv. campestris (Xcc) is a bacterium that causes black rot of crucifers. The greatest losses of brassica crop production usually result from seed-borne infection, but carry-over of inoculum in field soil may also be possible. The aim of this study was to monitor persistence of Xcc in field soil in central Europe using a conventional PCR assay with hrpF primers and a two-step nested real-time PCR assay using Zur primers. The work has demonstrated that nested real-time PCR can be used to improve the analytical sensitivity for detection of Xcc in soil compared to conventional PCR, and that Xcc may persist in soil for up to two years following an infected brassica crop in central European climatic conditions.

Department of Fruit Science Mendel University in Brno Valticka 337 691 44 Lednice Czech Republic

Department of Plant Biology and Biotechnology University of Agriculture in Krakow AL 29 Listopada 54 31 425 Krakow Poland

Department of Vegetable Science and Floriculture Mendel University in Brno Valticka 337 691 44 Lednice Czech Republic

Mendeleum Institute of Genetics Mendel University in Brno Valticka 334 691 44 Lednice Czech Republic

Plant Health Solutions Ltd 20 Beauchamp Road Warwick CV34 5NU UK

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