Understanding the molecular and biochemical basis of egg development is a central topic in mosquito reproductive biology. Lipids are a major source of energy and building blocks for the developing ovarian follicles. Ultra-High Resolution Mass Spectrometry (UHRMS) combined with in vivo metabolic labeling of follicle lipids with deuterated water (2H2O) can provide unequivocal identification of de novo lipid species during ovarian development. In the present study, we followed de novo triglyceride (TG) dynamics during the ovarian previtellogenic (PVG) stage (2-7 days post-eclosion) of female adult Aedes aegypti. The incorporation of stable isotopes from the diet was evaluated using liquid chromatography (LC) in tandem with the high accuracy (< 0.3 ppm) and high mass resolution (over 1 M) of a 14.5 T Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (14.5 T FT-ICR MS) equipped with hexapolar detection. LC-UHRMS provides effective lipid class separation and chemical formula identification based on the isotopic fine structure. The monitoring of stable isotope incorporation into de novo incorporated TGs suggests that ovarian lipids are consumed or recycled during the PVG stage, with variable time dynamics. These results provide further evidence of the complexity of the molecular mechanism of follicular lipid dynamics during oogenesis in mosquitoes.

Biomolecular Science Institute Florida International University Miami FL USA

Department of Biology Florida International University Miami FL USA

Department of Chemistry and Biochemistry Florida International University 11200 SW 8th St AHC4 233 Miami FL 33199 USA

Institute of Parasitology Biology Centre CAS Ceske Budejovice Czech Republic

Institute of Zoology Slovak Academy of Sciences Dubravska cesta 9 84506 Bratislava Slovakia

National High Magnetic Field Laboratory Florida State University Tallahassee FL USA

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Sci Rep. 2021 Aug 5;11(1):16319. doi: 10.1038/s41598-021-95696-y. PubMed

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