Surface plasmon resonance imaging biosensors have a number of advantages that make them superior to other analytical methods. These include the possibility of label-free detection, speed and high sensitivity to low protein concentrations. The aim of this study was to create and analyze biochips, with the help of which it is possible to test cystatin C in patient urine samples and compare the results with the one-time traditional ELISA method. The main advantage of the surface plasmon resonance imaging method is the possibility of repeated measurements over a long period of time in accordance with clinical practice. The surface of the biochip was spotted with anticystatin C and a negative control of mouse IgG at a ratio of 1:1. The aforementioned biochip was first verified using standard tests and then with patient samples, which clearly confirmed the required sensitivity even for very low concentrations of cystatin C.

Faculty of Applied Informatics Tomas Bata University in Zlin Nad Stranemi 4511 Zlin 760 05 Czech Republic

Faculty of Safety Engineering VSB Technical University of Ostrava Lumirova 13 Ostrava Vyskovice 700 30 Czech Republic

Nanotechnology Centre VSB Technical University of Ostrava 17 Listopadu 15 Ostrava Poruba 708 33 Czech Republic

References provided by Crossref.org