AIM: To test a novel method of assessment of platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions. METHODS: We developed a fluidic device that mimics blood flow in vessels. The method of detection of platelet adhesion is based on recording of a scattered laser light signal from a fibrinogen-covered surface. Testing was performed in platelet-rich plasma (PRP) and whole blood of healthy volunteers. Control measurements were performed, followed by tests with inhibition of platelet GPIIa/IIIb and GPIb receptors. Then, the same testing sequence was performed in whole blood of persons with autoimmune thrombocytopenia and type 3 von Willebrand disease. RESULTS: The change in intensity of scattered light was 2.7 (2.4; 4.1) times higher in whole blood (0.2 ± 0.08V, n = 7) than in PRP (0.05 ± 0.02 V, n = 7), p < 0.01. The blocking of GP IIb/IIIa receptors decreased the intensity of scattered light to 8.5 (6.5;12)%; the blocking of GPIb receptors decreased it to 34 (23;58)%, p < 0.01. In the whole blood of a person with autoimmune thrombocytopenia, the inhibition of GPIb receptors decreased platelet adhesion, but no effect was observed in type 3 von Willebrand disease. Inhibition of platelet GPIIb/IIIa receptors alone or combined inhibition of GPIb and GPIIb/IIIa receptors resulted in almost total suppression of adhesion in both cases. CONCLUSION: Our system effectively registers platelet adhesion to a fibrinogen-coated surface under controlled-flow conditions and may successfully be applied to the investigation of platelet adhesion kinetics.

1st Department of Internal Medicine Faculty of Medicine Comenius University and University Hospital Bratislava Slovakia

2nd Department of Internal Medicine Faculty of Medicine Masaryk University and St Anne´s University Hospital Brno Czech Republic

2nd Department of Surgery Faculty of Medicine Masaryk University and St Anne´s University Hospital Brno Czech Republic

Center for Translational Research and Systems Medicine Victor Babes University of Medicine and Pharmacy Timisoara Romania

Department of Functional Sciences Victor Babes University of Medicine and Pharmacy Timisoara Romania

Faculty of Medicine University of Oviedo and Central University Hospital of Asturias Oviedo Spain

Institute of Biomedical Problems of Russian Academy of Sciences Moscow Russia

National Medical Research Centre for Cardiology of the Ministry of Health of the Russian Federation Moscow Russia

National Research Center for Hematology of the Ministry of Health of the Russian Federation Moscow Russia

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