Prevalence of high-risk human papillomavirus DNA and mRNA and its association with abnormal anal cytology in the Czech male anal cancer screening cohort
Language English Country United States Media print-electronic
Document type Journal Article
Grant support
AZV17-31777A
Ministry of Health of the Czech Republic
PubMed
34529895
DOI
10.1002/dc.24873
Knihovny.cz E-resources
- Keywords
- Czech MSM, anal cancer screening, anal cytology, high-risk human papillomavirus DNA, high-risk human papillomavirus mRNA,
- MeSH
- Early Detection of Cancer statistics & numerical data MeSH
- DNA, Viral genetics MeSH
- Adult MeSH
- Homosexuality, Male genetics statistics & numerical data MeSH
- Papillomavirus Infections epidemiology pathology virology MeSH
- Middle Aged MeSH
- Humans MeSH
- RNA, Messenger genetics MeSH
- Young Adult MeSH
- Anus Neoplasms genetics pathology MeSH
- Prevalence MeSH
- Sexual and Gender Minorities statistics & numerical data MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Young Adult MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- DNA, Viral MeSH
- RNA, Messenger MeSH
BACKGROUND: Anal cancer (AC) screening is justified in high-risk populations, particularly HIV-positive men having sex with men (MSM). HR-HPV testing could improve the efficiency of cytologically based screening of AC, as in the screening of biologically analogical cervical cancer. The specificity of HR-HPV testing is influenced by the prevalence of HR-HPV infection in the screened population. Reported anal HR-HPV DNA prevalence in MSM is high, but HR-HPV mRNA reflects rather long-term infections and is more specific for high-grade lesions. However, no data were published about HR-HPV DNA and mRNA prevalence in the Czech AC screening population. METHOD: Results of liquid-based anal cytology of 203 predominantly HIV-positive MSM from the Czech AC screening cohort were correlated with results of DNA and E6/E7 mRNA testing of 14 HR-HPV types, and HPV16 genotyping. Eighty-one MSM underwent a standard anoscopy. RESULTS: A total of 109 (53.7%) samples had abnormal cytology, with 12 (5.9%) ASC-H/HSIL, 67 (33.0%) samples cytologically negative, and 27 (13.3%) unsatisfactory. HR-HPV DNA was detected in 134 (66.0%) and HR-HPV RNA in 72 (35.5%) anal smears. HR-HPV mRNA and HPV16 mRNA positivity were associated with abnormal cytology (p = .0037, p = .0021). No significant association was found between HR-HPV DNA or HPV16 DNA positivity and abnormal cytology. No high-grade lesions were revealed by anoscopy. CONCLUSION: Prevalence of anal HR-HPV DNA among Czech MSM is high, however, the prevalence of HR-HPV mRNA is half and associated with abnormal cytology. Our results indicate an increased efficiency of cytological screening when combined with HR-HPV mRNA testing.
Biomedical Center of the Faculty of Medicine in Pilsen Charles University Pilsen Czech Republic
Biopticka Laborator s r o Pilsen Czech Republic
Global Change Research Institute Czech Academy of Science Brno Czech Republic
Sikl's Department of Pathology University Hospital in Pilsen Pilsen Czech Republic
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