Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
34610315
DOI
10.1016/j.celrep.2021.109797
PII: S2211-1247(21)01257-2
Knihovny.cz E-resources
- Keywords
- Lipidomics, endocannabinoid signaling, enriched environment, multiomics, synaptic junctions,
- MeSH
- Amidohydrolases metabolism MeSH
- Receptors, AMPA metabolism MeSH
- Endocannabinoids metabolism MeSH
- Hippocampus cytology metabolism MeSH
- Rats MeSH
- Lipids analysis MeSH
- Lipid Metabolism * genetics MeSH
- Monoacylglycerol Lipases metabolism MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Rats, Wistar MeSH
- Proteome analysis MeSH
- Proteomics methods MeSH
- Signal Transduction * genetics MeSH
- Synapses metabolism MeSH
- Tandem Mass Spectrometry MeSH
- Chromatography, High Pressure Liquid MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Amidohydrolases MeSH
- Receptors, AMPA MeSH
- Endocannabinoids MeSH
- fatty-acid amide hydrolase MeSH Browser
- Lipids MeSH
- Monoacylglycerol Lipases MeSH
- Proteome MeSH
Membrane lipids and their metabolism have key functions in neurotransmission. Here we provide a quantitative lipid inventory of mouse and rat synaptic junctions. To this end, we developed a multiomics extraction and analysis workflow to probe the interplay of proteins and lipids in synaptic signal transduction from the same sample. Based on this workflow, we generate hypotheses about novel mechanisms underlying complex changes in synaptic connectivity elicited by environmental stimuli. As a proof of principle, this approach reveals that in mice exposed to an enriched environment, reduced endocannabinoid synthesis and signaling is linked to increased surface expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) in a subset of Cannabinoid-receptor 1 positive synapses. This mechanism regulates synaptic strength in an input-specific manner. Thus, we establish a compartment-specific multiomics workflow that is suitable to extract information from complex lipid and protein networks involved in synaptic function and plasticity.
Leibniz Institut für Analytische Wissenschaften ISAS e 5 44227 Dortmund Germany
RG Neuroplasticity Leibniz Institute for Neurobiology 39118 Magdeburg Germany
University of Pardubice Department of Analytical Chemistry CZ 532 10 Pardubice Czech Republic
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