Automated Time-Lapse Imaging and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical-Stage Confocal Microscopy
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Arabidopsis, Automation, Confocal microscopy, Cytokinesis, Laser ablation, Root meristem, Time-lapse imaging,
- MeSH
- Arabidopsis * MeSH
- Cell Division MeSH
- Time-Lapse Imaging MeSH
- Microscopy, Confocal MeSH
- Humans MeSH
- Meristem MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The analysis of dynamic cellular processes such as plant cytokinesis stands and falls with live-cell time-lapse confocal imaging. Conventional approaches to time-lapse imaging of cell division in Arabidopsis root tips are tedious and have low throughput. Here, we describe a protocol for long-term time-lapse simultaneous imaging of multiple root tips on a vertical-stage confocal microscope with automated root tracking. We also provide modifications of the basic protocol to implement this imaging method in the analysis of genetic, pharmacological or laser ablation wounding-mediated experimental manipulations. Our method dramatically improves the efficiency of cell division time-lapse imaging by increasing the throughput, while reducing the person-hour requirements of such experiments.
Department of Experimental Plant Biology Faculty of Science Charles University Prague Czechia
Department of Plant Biotechnology and Bioinformatics Ghent University Ghent Belgium
Institute of Science and Technology Austria Klosterneuburg Austria
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