Comparative assessment of immunochromatography and ELISA diagnostic tests for HBsAg detection in PCR-confirmed HBV infection
Jazyk angličtina Země Mexiko Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články
PubMed
34776394
DOI
10.1016/j.rgmxen.2021.11.007
PII: S2255-534X(21)00122-5
Knihovny.cz E-zdroje
- Klíčová slova
- Antígeno de superficie de hepatitis B, Ensayo inmunoabsorbente ligado a enzimas, Enzyme Linked Immunosorbent Assays, Hepatitis B Surface Antigen, Hepatitis B virus, Immunochromatography, Inmunocromatografía, Polymerase chain reaction, Reacción en cadena de la polimerasa, Virus de la hepatitis B,
- MeSH
- chromatografie afinitní MeSH
- diagnostické testy rutinní MeSH
- ELISA MeSH
- hepatitida B - antigeny povrchové * MeSH
- hepatitida B * diagnóza MeSH
- lidé MeSH
- novorozenec MeSH
- polymerázová řetězová reakce MeSH
- virus hepatitidy B genetika MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- novorozenec MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Geografické názvy
- Írán MeSH
- Názvy látek
- hepatitida B - antigeny povrchové * MeSH
INTRODUCTION AND AIMS: Viral hepatitis, which appears most frequently at birth or during childhood, is a disease whose transmission routes include tears, bile, sexual fluids, sweat, milk, urine, feces, and saliva. The aim of the present study was to analyze the specificity of the immunochromatographic and ELISA diagnostic tests for hepatitis B surface antigen and compare them with PCR testing. MATERIALS AND METHODS: The study sample was made up of 140 men and 60 women referred to the Urmia Medical University hospital to undergo PCR testing for HBV diagnosis. The ELISA test was performed using the Pioneer Medicine Company kit (Tehran, Iran). RESULTS: The results of the HBs-Ag rapid test and the ELISA test were compared with the PCR test. The HBs-Ag rapid test had 97% sensitivity and 91% specificity, whereas the ELISA test had 78% sensitivity and 76% specificity. DISCUSSION AND CONCLUSION: According to our results, the immunochromatographic test was accurate for diagnosing HBs-Ag in blood and the ELISA test had acceptable sensitivity and specificity, compared with PCR testing.
Departamento de Bioquímica Clínica Escuela de Medicina de Urmia Urmia Iran
Departamento de Biotecnología Microbiana Universidad Islámica Azad Urmia Iran
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