Trueperella (T.) bernardiae is a well-known bacterial pathogen in infections of humans, rarely in animals. In the present study, five T. bernardiae isolates, isolated from five Peking ducks of four different farms, were identified by phenotypic properties, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, and genotypically by sequencing the 16S ribosomal RNA (rRNA) gene, the superoxide dismutase A encoding gene sodA, and the glyceraldehyde-3-phosphate dehydrogenase encoding gene gap. In addition, the T. bernardiae isolates could be identified with a newly developed loop-mediated isothermal amplification (LAMP) assay based on the gyrase encoding housekeeping gene gyrA. All these tests clearly identified the T. bernardiae isolates to the species level. However, the detection of the specific gene gyrA with the newly designed LAMP assay appeared with a high sensitivity and specificity, and could help to identify this bacterial species in human and animal infections in future. The importance of the T. bernardiae isolates for the clinical condition of the ducks and for the problems at farm level remains unclear.

Hygiene and Zoonoses Department Faculty of Veterinary Medicine Mansoura University Elgomhoria Street 60 Mansoura 35516 Egypt

Institut für Hygiene und Infektionskrankheiten der Tiere Justus Liebig Universität Gießen Frankfurterstraße 85 91 35392 Gießen Germany

Institute for Animal Nutrition University of Veterinary Medicine Hannover Foundation Bischofsholer Damm 15 30173 Hannover Germany

Institute of Food Quality and Food Safety University of Veterinary Medicine Hannover Bischofsholer Damm 15 30173 Hannover Germany

Ripac Labor GmbH Am Mühlenberg 11 14476 Potsdam Germany

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