Lung cancer represents one of the leading worldwide causes of cancer death, but the pathobiochemistry of this disease is still not fully understood. Here we characterize the lipidomic and metabolomic profiles of the tumor and surrounding normal tissues for 23 patients with non-small cell lung cancer. In total, 500 molecular species were identified and quantified by a combination of the lipidomic shotgun tandem mass spectrometry (MS/MS) analysis and the targeted metabolomic approach using liquid chromatography (LC) - MS/MS. The statistical evaluation includes multivariate and univariate methods with the emphasis on paired statistical approaches. Our research revealed significant changes in several biochemical pathways related to the central carbon metabolism, acylcarnitines, dipeptides as well as the disruption in the lipid metabolism observed mainly for glycerophospholipids, sphingolipids, and cholesteryl esters.

Palacký University Olomouc Faculty of Medicine and Dentistry Hněvotínská 5 77900 Olomouc Czech Republic

Regional Hospital Pardubice Surgery Clinic Kyjevská 44 53203 Pardubice Czech Republic

University of Pardubice Faculty of Chemical Technology Department of Analytical Chemistry Studentská 573 53210 Pardubice Czech Republic

Citace poskytuje Crossref.org

Improved preanalytical workflow for pancreatic tissue lipidomics: insights into lipid stability and polar lipid recovery

Comprehensive metabolomic/lipidomic characterization of patients with mitochondrial ATP synthase, short-chain acyl-CoA dehydrogenase and combined variant deficiencies