A novel, eco-friendly, and biocompatible method was applied to form silver nanoparticles (AgNPs) in great water dock (Lapathi radix) (KB) and pu-erh (Camellia sinensis) (PE) extracts. The surface plasma resonance peak of green synthesized AgNPs at 451.8 nm for AgNPs+KB and 440.8 nm for AgNPs+PE was observed via spectral analysis of UV absorbance. In this study, double-layered biopolymer films (FUR/CHIT+HGEL) with AgNPs incorporated in KB solution (AgNPs+KB) and AgNPs in PE solution (AgNPs+PE), were successfully prepared using the casting method. The SEM, XRD, zeta potential and size analyses confirmed the presence of AgNP in the films. The addition of AgNPs in plant extracts improved antimicrobial and antioxidant activity and thermal stability, whereas WVTR experienced a decrease. The nanocomposite films' orange-brown colour may aid in the protection of food products against UV rays. The composite films demonstrated antibacterial activity against food-borne pathogens and may offer potential in food packaging applications.

Department of Animal Product Technology Faculty of Food Technology University of Agriculture ul Balicka 122 30 149 Kraków Poland

Department of Chemistry University of Agriculture ul Balicka 122 30 149 Kraków Poland

Department of Food Analysis and Evaluation of Food Quality University of Agriculture ul Balicka 122 30 149 Krakow Poland

Department of Infections Control and Mycology Jagiellonian University Medical College ul Czysta 18 31 121 Kraków Poland

Department of Inorganic Chemistry Faculty of Science Palacky University 17 Listopadu 12 CZ 771 46 Olomouc Czech Republic

Department of Product Packaging Cracow University of Economics ul Rakowicka 27 30 510 Kraków Poland

Jerzy Haber Institute of Catalysis and Surface Chemistry Polish Academy of Sciences ul Niezapominajek 8 30 239 Kraków Poland

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