Mushrooms supplementation with iron (Fe) is usually limited, and therefore it would be beneficial to search for other vital elements able to improve the process. The aim of this study was to verify a possible interaction between Fe and calcium (Ca) to estimate the role of the addition of the latter metal to stimulate Fe accumulation in Pholiota nameko. Additionally, an analysis of phenolic compounds and low molecular weight organic acids (LMWOAs) was performed. The increase of Fe concentration in the substrate caused a significantly higher accumulation of this metal in P. nameko. The addition of Ca (5 or 10 mM) stimulated Fe accumulation, just as Fe concentration in the substrate stimulated Ca accumulation, which pointed to a synergism between these metals. The obtained results show that the presence of Fe in the substrate may also promote K, Mg, Mn, Na, P, and S accumulation. In contrast, the addition of Ca stimulates and/or inhibits their content in fruit bodies. The phenolic and organic acids profile was poor. Only gallic, 4-hydroxybenzoic, sinapic and syringic acids (phenolics), as well as citric and succinic acids (LMWOAs), were quantified in some combinations in P. nameko fruiting bodies.

Department of Applied Chemistry Faculty of Agriculture University of South Bohemia 370 04 České Budějovice Czech Republic

Department of Chemistry Faculty of Forestry and Wood Technology Poznań University of Life Sciences Wojska Polskiego 75 60 625 Poznań Poland

Department of Environmental Medicine Poznań University of Medical Sciences Rokietnicka 8 60 806 Poznań Poland

Department of Mathematical and Statistical Methods Poznań University of Life Sciences Wojska Polskiego 28 60 637 Poznań Poland

Department of Vegetable Crops Faculty of Agriculture Horticulture and Bioengineering Poznań University of Life Sciences Dąbrowskiego 159 60 594 Poznań Poland

Faculty of Chemistry Adam Mickiewicz University in Poznań Uniwersytetu Poznańskiego 8 61 614 Poznań Poland

Integrated Science Association Rokietnicka 8 60 806 Poznań Poland

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