In this study, a simple and green strategy was reported to prepare bimetallic nanoparticles (NPs) by the combination of zinc oxide (ZnO) and copper oxide (CuO) using Sambucus nigra L. extract. The physicochemical properties of these NPs such as crystal structure, size, and morphology were studied by X-ray diffraction (XRD), field emission gun scanning electron microscopy (FEG-SEM), and transmission electron microscopy (TEM). The results suggested that these NPs contained polygonal ZnO NPs with hexagonal phase and spherical CuO NPs with monoclinic phase. The anticancer activity of the prepared bimetallic NPs was evaluated against lung and human melanoma cell lines based on MTT assay. As a result, the bimetallic ZnO/CuO NPs exhibited high toxicity on melanoma cancer cells while their toxicity on lung cancer cells was low.

Department of Basic Engineering Science Faculty of Engineering Menoufia University Shebin El Kom 32511 Egypt

Department of Biology Ardabil Branch Islamic Azad University Ardabil Iran

Department of Biology Islamic Azad University Tehran Medical Branch Tehran Iran

Department of Biotechnology Shahid Bahonar University of Kerman Kerman Iran

Department of Mathematics College of Science and Humanities in Al Kharj Prince Sattam Bin Abdulaziz University Al Kharj 11942 Saudi Arabia

Department of Medical Biotechnology School of Advanced Technologies in Medicine Shahid Beheshti University of Medical Sciences Tehran Iran

Faculty of Chemical and Petroleum Engineering Sahand University of Technology Tabriz Iran

Faculty of Nursing and Midwifery Zabol University of Medical Sciences Zabol Iran

Faculty of Pharmacy and Pharmaceutical Sciences Isfahan University of Medical Sciences Isfahan Iran

Institute of Macromolecular Chemistry Czech Academy of Sciences Heyrovskeho nam 2 162 06 Prague 6 Czech Republic

Mechanical Engineering Department University of Technology Baghdad Iraq

Noncommunicable Diseases Research Center Bam University of Medical Sciences Bam Iran

Polymer Institute of the Slovak Academy of Sciences Dúbravská Cesta 9 845 41 Bratislava Slovakia

School of Mechatronic Engineering Xi'an Technological University Xi'an 710021 China

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