A small portion of plastid transcripts is polyadenylated in the flagellate Euglena gracilis
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24492004
DOI
10.1016/j.febslet.2014.01.034
PII: S0014-5793(14)00063-5
Knihovny.cz E-zdroje
- Klíčová slova
- EST, Euglenozoa, Plastid, Polyadenylation, Quantitative PCR, Trans-splicing,
- MeSH
- Euglena gracilis genetika metabolismus účinky záření MeSH
- exprimované sekvenční adresy MeSH
- genom plastidový MeSH
- messenger RNA genetika metabolismus MeSH
- plastidy genetika metabolismus MeSH
- polyadenylace * MeSH
- protozoální geny MeSH
- RNA protozoální genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- messenger RNA MeSH
- RNA protozoální MeSH
Euglena gracilis possesses secondary plastids of green algal origin. In this study, E. gracilis expressed sequence tags (ESTs) derived from polyA-selected mRNA were searched and several ESTs corresponding to plastid genes were found. PCR experiments failed to detect SL sequence at the 5'-end of any of these transcripts, suggesting plastid origin of these polyadenylated molecules. Quantitative PCR experiments confirmed that polyadenylation of transcripts occurs in the Euglena plastids. Such transcripts have been previously observed in primary plastids of plants and algae as low-abundance intermediates of transcript degradation. Our results suggest that a similar mechanism exists in secondary plastids.
Department of Biology and Ecology Faculty of Science University of Ostrava Ostrava Czech Republic
Department of Genetics Faculty of Natural Sciences Comenius University Bratislava Slovakia
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