A small portion of plastid transcripts is polyadenylated in the flagellate Euglena gracilis
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24492004
DOI
10.1016/j.febslet.2014.01.034
PII: S0014-5793(14)00063-5
Knihovny.cz E-resources
- Keywords
- EST, Euglenozoa, Plastid, Polyadenylation, Quantitative PCR, Trans-splicing,
- MeSH
- Euglena gracilis genetics metabolism radiation effects MeSH
- Expressed Sequence Tags MeSH
- Genome, Plastid MeSH
- RNA, Messenger genetics metabolism MeSH
- Plastids genetics metabolism MeSH
- Polyadenylation * MeSH
- Genes, Protozoan MeSH
- RNA, Protozoan genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- RNA, Messenger MeSH
- RNA, Protozoan MeSH
Euglena gracilis possesses secondary plastids of green algal origin. In this study, E. gracilis expressed sequence tags (ESTs) derived from polyA-selected mRNA were searched and several ESTs corresponding to plastid genes were found. PCR experiments failed to detect SL sequence at the 5'-end of any of these transcripts, suggesting plastid origin of these polyadenylated molecules. Quantitative PCR experiments confirmed that polyadenylation of transcripts occurs in the Euglena plastids. Such transcripts have been previously observed in primary plastids of plants and algae as low-abundance intermediates of transcript degradation. Our results suggest that a similar mechanism exists in secondary plastids.
Department of Biology and Ecology Faculty of Science University of Ostrava Ostrava Czech Republic
Department of Genetics Faculty of Natural Sciences Comenius University Bratislava Slovakia
References provided by Crossref.org
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