Analysis of catRABC operon for catechol degradation from phenol-degrading Rhodococcus erythropolis
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- bakteriální geny * MeSH
- bakteriální proteiny genetika metabolismus MeSH
- DNA vazebné proteiny genetika metabolismus MeSH
- fenol metabolismus MeSH
- genetická transkripce * MeSH
- intergenová DNA genetika MeSH
- intramolekulární lyasy genetika metabolismus MeSH
- isomerasy dvojných vazeb mezi uhlíky genetika metabolismus MeSH
- katechol-1,2-dioxygenasa genetika metabolismus MeSH
- katecholy metabolismus MeSH
- kyselina jantarová MeSH
- molekulární sekvence - údaje MeSH
- multigenová rodina genetika MeSH
- operon genetika MeSH
- promotorové oblasti (genetika) * MeSH
- receptorový protein pro cyklický AMP genetika MeSH
- represorové proteiny genetika metabolismus MeSH
- Rhodococcus enzymologie genetika MeSH
- sekvence nukleotidů MeSH
- sekvenční seřazení MeSH
- transkripční faktory genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bakteriální proteiny MeSH
- catechol MeSH Prohlížeč
- CatR protein, bacteria MeSH Prohlížeč
- DNA vazebné proteiny MeSH
- fenol MeSH
- intergenová DNA MeSH
- intramolekulární lyasy MeSH
- isomerasy dvojných vazeb mezi uhlíky MeSH
- katechol-1,2-dioxygenasa MeSH
- katecholy MeSH
- kyselina jantarová MeSH
- muconate cycloisomerase MeSH Prohlížeč
- muconolactone delta-isomerase MeSH Prohlížeč
- receptorový protein pro cyklický AMP MeSH
- represorové proteiny MeSH
- transkripční faktory MeSH
The gene cluster catRABC, involved in catechol degradation, was isolated from Rhodococcus erythropolis CCM2595. The genes catA, catB, catC, and the divergently transcribed catR code for catechol 1,2-dioxygenase, cis,cis-muconate cycloisomerase, muconolactone isomerase, and an IclR-type transcriptional regulator, respectively. Measurements of catechol 1,2-dioxygenase activity showed that the expression of catA is induced by phenol but not by catechol or cis,cis-muconate. The activity of catechol 1,2-dioxygenase was repressed by succinate, but no repression by glucose was observed. The transcription start points of catA and catR were determined by primer extension analysis, and the respective promoters (P-catA and P-catR) were thus localized. Measurements of promoter activity during batch cultivation using transcriptional fusion with the gfpuv reporter gene showed that expression of the catR-catABC operon is regulated at the level of transcription. Both P-catR and P-catA are repressed by CatR, and the induction of P-catA by phenol is maintained in the absence of the repressor (in R. erythropolis DeltacatR). Two different potential binding sites for the IclR-type regulator and a recognition site for the cyclic AMP receptor protein (CRP) were identified within the intergenic region between catR and catA.
Citace poskytuje Crossref.org
Genome Sequence of Rhodococcus erythropolis Strain CCM2595, a Phenol Derivative-Degrading Bacterium
General and molecular microbiology and microbial genetics in the IM CAS
