Analysis of catRABC operon for catechol degradation from phenol-degrading Rhodococcus erythropolis
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Genes, Bacterial * MeSH
- Bacterial Proteins genetics metabolism MeSH
- DNA-Binding Proteins genetics metabolism MeSH
- Phenol metabolism MeSH
- Transcription, Genetic * MeSH
- DNA, Intergenic genetics MeSH
- Intramolecular Lyases genetics metabolism MeSH
- Carbon-Carbon Double Bond Isomerases genetics metabolism MeSH
- Catechol 1,2-Dioxygenase genetics metabolism MeSH
- Catechols metabolism MeSH
- Succinic Acid MeSH
- Molecular Sequence Data MeSH
- Multigene Family genetics MeSH
- Operon genetics MeSH
- Promoter Regions, Genetic * MeSH
- Cyclic AMP Receptor Protein genetics MeSH
- Repressor Proteins genetics metabolism MeSH
- Rhodococcus enzymology genetics MeSH
- Base Sequence MeSH
- Sequence Alignment MeSH
- Transcription Factors genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
- catechol MeSH Browser
- CatR protein, bacteria MeSH Browser
- DNA-Binding Proteins MeSH
- Phenol MeSH
- DNA, Intergenic MeSH
- Intramolecular Lyases MeSH
- Carbon-Carbon Double Bond Isomerases MeSH
- Catechol 1,2-Dioxygenase MeSH
- Catechols MeSH
- Succinic Acid MeSH
- muconate cycloisomerase MeSH Browser
- muconolactone delta-isomerase MeSH Browser
- Cyclic AMP Receptor Protein MeSH
- Repressor Proteins MeSH
- Transcription Factors MeSH
The gene cluster catRABC, involved in catechol degradation, was isolated from Rhodococcus erythropolis CCM2595. The genes catA, catB, catC, and the divergently transcribed catR code for catechol 1,2-dioxygenase, cis,cis-muconate cycloisomerase, muconolactone isomerase, and an IclR-type transcriptional regulator, respectively. Measurements of catechol 1,2-dioxygenase activity showed that the expression of catA is induced by phenol but not by catechol or cis,cis-muconate. The activity of catechol 1,2-dioxygenase was repressed by succinate, but no repression by glucose was observed. The transcription start points of catA and catR were determined by primer extension analysis, and the respective promoters (P-catA and P-catR) were thus localized. Measurements of promoter activity during batch cultivation using transcriptional fusion with the gfpuv reporter gene showed that expression of the catR-catABC operon is regulated at the level of transcription. Both P-catR and P-catA are repressed by CatR, and the induction of P-catA by phenol is maintained in the absence of the repressor (in R. erythropolis DeltacatR). Two different potential binding sites for the IclR-type regulator and a recognition site for the cyclic AMP receptor protein (CRP) were identified within the intergenic region between catR and catA.
References provided by Crossref.org
Genome Sequence of Rhodococcus erythropolis Strain CCM2595, a Phenol Derivative-Degrading Bacterium
General and molecular microbiology and microbial genetics in the IM CAS
