Approaches using microsatellite markers are considered the gold standard for modern population genetic studies. However, although they have found application in research into various platyhelminth taxa, they remained substantially underutilized in the study of monogeneans. In the present study, a newly developed set of 24 microsatellite markers was used to investigate the genetic diversity of the generalist monogenean species Dactylogyrus vistulae. The analyzed parasite specimens were collected from 13 cyprinoid species from 11 sites in the Apennine and Balkan peninsulas. A total of 159 specimens were genotyped at each of the loci and the number of alleles per locus ranged from 2 to 16, with a mean number of 6.958 alleles per locus. Exceptionally high genetic diversity was observed among D. vistulae individuals in the southern Balkans (mean N A per locus = 3.917), suggesting that generalist D. vistulae expanded from the south to the north in the Balkans and later into central Europe. The initial clustering analysis divided all investigated specimens into three major clusters; however, the results of the subsequent analyses revealed the existence of various subpopulations, suggesting that the population structure of D. vistulae is associated with the diversification of their cyprinoid hosts. In addition, the partition of the parasite population was observed in regions of the sympatric occurrence of two host species, indicating that these hosts may represent a barrier for gene flow, even for generalist parasite species.

Department of Botany and Zoology Faculty of Science Masaryk University Brno Czech Republic

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Abell, R. , Thieme, M. L. , Revenga, C. , Bryer, M. , Kottelat, M. , Bogutskaya, N. , Coad, B. , Mandrak, N. , Balderas, S. C. , Bussing, W. , Stiassny, M. L. J. , Skelton, P. , Allen, G. R. , Unmack, P. , Naseka, A. , Ng, R. , Sindorf, N. , Robertson, J. , Armijo, E. , … Petry, P. (2008). Freshwater ecoregions of the world: A new map of biogeographic units for freshwater biodiversity conservation. BioScience, 58, 403–414. 10.1641/B580507 DOI

Albrecht, C. , & Wilke, T. (2008). Ancient Lake Ohrid: Biodiversity and evolution. Hydrobiologia, 615, 103–140. 10.1007/s10750-008-9558-y DOI

Antonio Baeza, J. , Sepúlveda, F. A. , & Teresa González, M. (2019). The complete mitochondrial genome and description of a new cryptic species of Benedenia Diesing, 1858 (Monogenea: Capsalidae), a major pathogen infecting the yellowtail kingfish Seriola lalandi Valenciennes in the South‐East Pacific. Parasites & Vectors, 12, 490. 10.1186/s13071-019-3711-5 PubMed DOI PMC

Aydogdu, A. , Altunel, F. N. , & Yildirimhan, H. S. (2001). Occurrence of helminths in Chub, Leuciscus cephalus, of the Doganci (Bursa) dam lake, Turkey. Bulletin of the European Association of Fish Pathologists, 21, 246–251.

Bazsalovicsová, E. , Králová‐Hromadová, I. , Juhásová, L. , Mikulíček, P. , Oravcová, A. , Minárik, G. , & Štefka, J. (2020). Comparative analysis of monozoic fish tapeworms Caryophyllaeus laticeps (Pallas, 1781) and recently described Caryophyllaeus chondrostomi Barčák, Oros, Hanzelová, Scholz, 2017, using microsatellite markers. Parasitology Research, 119, 3995–4004. 10.1007/s00436-020-06898-8 PubMed DOI

Benovics, M. , Desdevises, Y. , Šanda, R. , Vukić, J. , Scheifler, M. , Doadrio, I. , & Šimková, A. (2020). High diversity of fish ectoparasitic monogeneans (Dactylogyrus) in the Iberian Peninsula: a case of adaptive radiation? Parasitology, 147, 418–430. PubMed PMC

Benovics, M. , Desdevises, Y. , Šanda, R. , Vukić, J. , & Šimková, A. (2020). Cophylogenetic relationships between Dactylogyrus (Monogenea) ectoparasites and endemic cyprinoids of the north‐eastern European peri‐Mediterranean region. Journal of Zoological Systematics and Evolutionary Research, 58, 1–21.

Benovics, M. , Desdevises, Y. , Vukić, J. , Šanda, R. , & Šimková, A. (2018). The phylogenetic relationships and species richness of host‐specific Dactylogyrus parasites shaped by the biogeography of Balkan cyprinids. Scientific Reports, 8, 13006. 10.1038/s41598-018-31382-w PubMed DOI PMC

Benovics, M. , Francová, K. , Volta, P. , Dlapka, V. , & Šimková, A. (2021). Helminth communities of endemic cyprinoids of the Apennine Peninsula, with remarks on ectoparasitic monogeneans, and a description of four new Dactylogyrus Diesing, 1850 species. Parasitology, 2021, 1–16. PubMed PMC

Bianco, P. G. (1990). Potential role of the paleohistory of the Mediterranean and Parathethys basins on the early dispersal of Euro Mediterranean freshwater fishes. Ichthyological Exploration of Freshwaters, 1, 167–184.

Bordon, A. , Peyron, O. , Lezine, A. M. , Brewer, S. , & Fouache, E. (2009). Pollen‐inferred Late‐Glacial and Holocene climate in southern Balkans (Lake Maliq). Quaternary International, 200, 19–30. 10.1016/j.quaint.2008.05.014 DOI

Buj, I. , Marčić, Z. , Čaleta, M. , Šanda, R. , Geiger, M. F. , Freyhof, J. , & Vukić, J. (2017). Ancient connections among European rivers and watersheds revealed from the evolutionary history of the genus Telestes (Actinopterygii; Cypriniformes). PLoS One, 12, e0187366. PubMed PMC

Buj, I. , Vukić, J. , Šanda, R. , Perea, S. , Ćaleta, M. , Marčic, Z. , & Mrakovčić, M. (2010). Morphological comparison of bleaks (Alburnus, Cyprinidae) from the Adriatic Basin with the description of a new species. Folia Zoologica, 59, 129–141.

Criscione, C. D. , & Blouin, M. S. (2006). Minimal selfing, few clones, and no among‐host genetic structure in a hermaphroditic parasite with asexual larval propagation. Evolution, 60, 553–562. PubMed

Criscione, C. D. , Cooper, B. , & Blouin, M. S. (2006). Parasite genotypes identify source populations of migratory fish more accurately than fish genotypes. Ecology, 87, 823–828. PubMed

Criscione, C. D. , van Paridon, B. J. , Gilleard, J. S. , & Goater, C. P. (2020). Clonemate cotransmission supports a role for kin selection in a puppeteer parasite. Proceedings of the National Academy of Sciences, 117, 5970–5976. 10.1073/pnas.1922272117. PubMed DOI PMC

Criscione, C. D. , Vilas, R. , Paniagua, E. , & Blouin, M. S. (2011). More than meets the eye: detecting cryptic microgeographic population structure in a parasite with a complex life cycle. Molecular Ecology, 20, 2510–2524. 10.1111/j.1365-294X.2011.05113.x PubMed DOI

Dar, Y. , Vignoles, P. , Rondelaud, D. , & Dreyfuss, G. (2015). Role of lymnaeid snail Pseudosuccinea collumnela on the transmission of the liver fluke Fasciola hepatica in Egypt. Journal of Helminthology, 89, 699–706. PubMed

Doadrio, I. (1990). Phylogenetic relationships and classification of western Palearctic species of the genus Barbus (Osteichthyes, Cyprinidae). Aquatic Living Resources, 3, 265–282.

Earl, D. A. , & vonHoldt, B. M. (2012). STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conservation Genetic Resources, 4, 359–361. 10.1007/s12686-011-9548-7. DOI

Evanno, G. , Regnaut, S. , & Gould, J. (2005). Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology, 14, 2611–2620. 10.1111/j.1365-294X.2005.02553.x PubMed DOI

Falush, D. , Stephens, M. , & Pritchard, J. K. (2007). Inference of population structure using multilocus genotype data: dominant markers and null alleles. Molecular Ecology Notes, 7, 574–578. 10.1111/j.1471-8286.2007.01758.x PubMed DOI PMC

Faria, P. J. , Lazarus, C. M. , van Oosterhout, C. , Harris, P. D. , & Cable, J. (2011). First polymorphic microsatellites for the gyrodactylids (Monogenea), an important group of fish pathogens. Conservation Genetics Resources, 3, 177–180. 10.1007/s12686-010-9317-z DOI

Geiger, M. F. , Herder, F. , Monaghan, M. T. , Almada, V. , Barbieri, R. , Bariche, M. , Berrebi, P. , Bohlen, J. , Casal‐Lopez, M. , Delmastro, G. B. , Denys, G. P. J. , Dettai, A. , Doadrio, I. , Kalogianni, E. , Kärst, H. , Kottelat, M. , Kovačić, M. , Laporte, M. , Lorenzoni, M. , … Freyhof, J. (2014). Spatial heterogeneity in the Mediterranean biodiversity hotspot affects barcoding accuracy of its freshwater fishes. Molecular Ecology Resources, 14, 1210–1221. 10.1111/1755-0998.12257 PubMed DOI

Gibson, D. I. , Timofeeva, T. A. , & Gerasev, P. I. (1996). Catalogue of the nominal species of the monogeneans of genus Dactylogyrus Diesing, 1850 and their host genera. Systematic Parasitology, 35, 3–48.

Gower, C. M. , Gabrielli, A. F. , Sacko, M. , Dembelé, R. , Golan, R. , Emery, A. M. , & Webster, J. P. (2011). Population genetics of Schistosoma haematobium: development of novel microsatellite markers and their application to schistosomiasis control in Mali. Parasitology, 138, 978–994. PubMed

Hague, M. T. J. , & Routman, E. J. (2016). Does population size affect genetic diversity? A test of sympatric lizard species. Heredity, 116, 92–98. PubMed PMC

Hoffman, G. L. (1999). Parasites of North American freshwater fishes, 2nd edn. Cornell University Press.

Huyse, T. , Oeyen, M. , Larmuseau, M. H. D. , & Volckaert, F. A. M. (2017). Co‐phylogeographic study of the flatworm Gyrodactylus gondae and its goby host Pomatoschistus minutus . Parasitology International, 66, 119–125. 10.1016/j.parint.2016.12.008 PubMed DOI

Huyse, T. , & Volckaert, F. A. (2002). Identification of a host‐associated species complex using molecular and morphometric analyses, with the description of Gyrodactylus rugiensoides n. sp. (Gyrodactylidae, Monogenea). International Journal for Parasitology, 32, 907–919. 10.1016/S0020-7519(02)00026-7 PubMed DOI

Juhásová, L. , Králová‐Hromadová, I. , Bazsalovicsová, E. , Minárik, G. , Štefka, J. , Mikulíček, P. , Pálková, L. , & Pybus, M. (2016). Population structure and dispersal routes of an invasive parasite Fascioloides magna, in North America and Europe. Parasites & Vectors, 9, 547. 10.1186/s13071-016-1811-z PubMed DOI PMC

Khang, T. F. , Soo, O. Y. M. , Tan, W. B. , & Lim, L. H. S. (2016). Monogenean anchor morphometry: systematic value, phylogenetic signal, and evolution. PeerJ, 4, e1668. 10.7717/peerj.1668 PubMed DOI PMC

Kmentová, N. , Gelnar, M. , Mendlová, M. , Van Steenberge, M. , Koblmüller, S. , & Vanhove, M. P. M. (2016). Reduced host‐specificity in a parasite infecting non‐littoral Lake Tanganyika cichlids evidenced by intraspecific morphological and genetic diversity. Scientific Reports, 6, 39605. 10.1038/srep39605 PubMed DOI PMC

Kmentová, N. , Koblmüller, S. , Van Steenberge, M. , Artois, T. , Muterezi Bukinga, F. , Mulimbwa N'sibula, T. , Muzumani Risasi, D. , Masilya Mulungula, P. , Gelnar, M. , & Vanhove, M. P. M. (2020). Failure to diverge in African Great Lakes: the case of Dolicirroplectanum lacustre gen. nov., comb. nov. (Monogenea, Diplectanidae) infecting latid hosts. Journal of Great Lakes Research, 46, 1113–1130. 10.1016/j.jglr.2019.09.022 DOI

Kmentová, N. , Koblmüller, S. , Van Steenberge, M. , Raeymaekers, J. A. M. , Artois, T. , De Keyzer, E. L. R. , Milec, L. , Muterezi Bukinga, F. , Mulimbwa N'sibula, T. , Masilya Mulungula, P. , Ntakimazi, G. , Volckaert, F. A. M. , Gelnar, M. , & Vanhove, M. P. M. (2020). Weak population structure and recent demographic expansion of the monogenean parasite Kapentagyrus spp. infecting clupeid fishes of Lake Tanganyika, East Africa. International Journal for Parasitology, 50, 471–486. 10.1016/j.ijpara.2020.02.002 PubMed DOI

Kopelman, N. M. , Mayzel, J. , Jakobsson, M. , Rosenberg, N. A. , & Mayrose, I. (2015). CLUMPAK: a program for identifying clustering modes and packaging population structure inferences across K". Molecular Ecology Resources, 15, 1179–1191. PubMed PMC

Koskivaara, M. , & Valtonen, E. T. (1992). Dactylogyrus (Monogenea) communities on the gills of roach in three lakes in Central Finland. Parasitology, 104, 263–272.

Kottelat, M. , & Freyhof, J. (2007). Handbook of European freshwater fishes. Kottelat, Cornol and Freyhof.

Kuchta, R. , Řehulková, E. , Francová, K. , Scholz, T. , Morand, S. , & Šimková, A. (2020). Diversity of monogeneans and tapeworms in cypriniform fishes across two continents. International Journal for Parasitology, 50, 771–786. 10.1016/j.ijpara.2020.06.005 PubMed DOI

Louhi, K.‐R. , Karvonen, A. , Rellstab, C. , & Jokela, J. (2010). Is the population genetic structure of complex life cycle parasites determined by the geographic range of the most motile host? Infections, Genetics and Evolution, 10, 1271–1277. 10.1016/j.meegid.2010.08.013 PubMed DOI

Luo, H. Y. , Nie, P. , Zhang, Y. A. , Yao, W. J. , & Wang, G. T. (2003). Genetic differentiation in populations of the cestode Bothriocephalus acheilognathi (Cestoda, Pseudophyllidea) as revealed by eight microsatellite markers. Parasitology, 126, 493–501. PubMed

Mariniello, L. , Ortis, M. , D’Amelio, S. , & Petrarca, V. (2004). Morphometric variability between and within species of Ligophorus Euzet & Suriano, 1977 (Monogenea: Ancyrocephalidae) in the Mediterranean Sea. Systematic Parasitology, 57, 183–190. PubMed

Meglécz, E. , Costedoat, C. , Dubut, V. , Gilles, A. , Malausa, T. , Pech, N. , & Martin, J. F. (2010). QDD: a user‐friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics, 26, 403–404. PubMed

Mhaisen, F. T. , & Abdul‐Ameer, K. N. (2019). Checklists of Dactylogyrus species (Monogenea) from Fishes of Iraq. Biological and Applied Environmental Research, 3, 1–36.

Moravec, F. (2001). Checklist of the metazoan parasites of fishes of Czech Republic and Slovak Republic (1873–2000). Academia.

Palandačić, A. , Bravničar, J. , Zupančič, P. , Šanda, R. , & Snoj, A. (2015). Molecular data suggest a multispecies complex of Phoxinus (Cyprinidae) in the Western Balkan Peninsula. Molecular Phylogenetics and Evolution, 92, 118–123. 10.1016/j.ympev.2015.05.024 PubMed DOI

Peakall, R. , & Smouse, P. E. (2006). GenAlEx 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes, 6, 288–295. 10.1111/j.1471-8286.2005.01155.x PubMed DOI PMC

Peakall, R. , & Smouse, P. E. (2012). GenAlEx 6.5: genetic analysis in excel. Population genetic software for teaching and research—an update. Bioinformatics, 28, 2537–2539. PubMed PMC

Perea, S. , Böhme, M. , Zupančič, P. , Freyhof, J. , Šanda, R. , Özuluğ, M. , Abdoli, A. , & Doadrio, I. (2010). Phylogenetic relationships and biogeographical patterns in Circum‐Mediterranean subfamily Leuciscinae (Teleostei, Cyprinidae) inferred from both mitochondrial and nuclear data. BMC Evolutionary Biology, 10, 265. 10.1186/1471-2148-10-265 PubMed DOI PMC

Pettersen, R. A. , Junge, C. , Østbye, K. , Mo, T. A. , & Vøllestad, L. A. (2021). Genetic population structure of the monogenean parasite Gyrodactylus thymalli and its host European grayling (Thymallus thymallus) in a large Norwegian lake. Hydrobiologia, 848, 547–561. 10.1007/s10750-020-04431-7 DOI

Pielou, E. C. (1979). Biogeography. Wiley.

Pritchard, J. K. , Stephens, M. , & Donnelly, P. J. (2000). Inference of population structure using multilocus genotype data. Genetics, 155, 945–959. 10.1093/genetics/155.2.945 PubMed DOI PMC

Pugachev, O. N. , Gerasev, P. I. , Gussev, A. V. , Ergens, R. , & Khotenowsky, I. (2010). Guide to Monogenoidea of freshwater fish of Palearctic and Amur Regions. Ledizione‐Ledi Publishing.

Rahmouni, C. , Van Steenberge, M. , Vanhove, M. P. M. , & Šimková, A. (2021). Intraspecific morphological variation in Cichlidogyrus (Monogenea) parasitizing two cichlid hosts from Lake Tanganyika exhibiting different dispersal capacities. Hydrobiologia, 848(16), 3833–3845. 10.1007/s10750-020-04429-1 DOI

Řehulková, E. , Benovics, M. , & Šimková, A. (2020). Uncovering the diversity of monogeneans (Platyhelminthes) on endemic cypriniform fishes of the Balkan Peninsula: new species of Dactylogyrus and comments on their phylogeny and host‐parasite associations in a biogeographic context. Parasite, 27, 66. PubMed PMC

Řehulková, E. , Seifertová, M. , Přikrylová, I. , & Francová, K. (2018). Monogenea. In Scholz T., Vanhove M. P. M., Smit N., Jayasundera Z., & Gelnar M. (Eds.), A guide to the parasites of African freshwater fishes (pp. 185–243). AbcTaxa.

Rodríguez‐González, A. , Míguez‐Lozano, R. , Llopis‐Belenguer, C. , & Balbuena, J. A. (2015). Phenotypic plasticity in haptoral structures of Ligophorus cephali (Monogenea: Dactylogyridae) on the flathead mullet (Mugil cephalus): a geometric morphometric approach. International Journal for Parasitology, 45, 295–303. 10.1016/j.ijpara.2015.01.005 PubMed DOI

Schelkle, B. , Faria, P. J. , Johnson, M. B. , van Oosterhout, C. , & Cable, J. (2012). Mixed infections and hybridisation in monogenean parasites. PLoS One, 7, e39506. 10.1371/journal.pone.0039506 PubMed DOI PMC

Schönhuth, S. , Vukić, J. , Šanda, R. , Yang, L. , & Mayden, R. L. (2018). Phylogenetic relationships and classification of the Holarctic family Leuciscidae (Cypriniformes: Cyprinoidei). Molecular Phylogenetics and Evolution, 127, 781–799. 10.1016/j.ympev.2018.06.026 PubMed DOI

Schultheiss, R. , Albrecht, C. , Bossneck, U. , & Wilke, T. (2008). The neglected side of speciation in ancient lakes: Phylogeography of an in conspicuous mollusc taxon in lakes Ohrid and Prespa. Hydrobiologia, 615, 141–156. 10.1007/s10750-008-9553-3 DOI

Seifertová, M. , Vyskočilová, M. , Morand, S. , & Šimková, A. (2008). Metazoan parasites of freshwater cyprinid fish (Leuciscus cephalus): testing biogeography hypotheses of species diversity. Parasitology, 135, 1417–1435. PubMed

Šimková, A. , Benovics, M. , Rahmouni, I. , & Vukić, J. (2017). Host‐specific Dactylogyrus parasites revealing new insights on the historical biogeography of Northwest African and Iberian cyprinid fish. Parasites & Vectors, 10, 589. 10.1186/s13071-017-2521-x PubMed DOI PMC

Šimková, A. , Morand, S. , Jobet, E. , Gelnar, M. , & Verneau, O. (2004). Molecular phylogeny of congeneric monogenean parasites (Dactylogyrus): A case of intrahost speciation. Evolution, 58, 1001–1018. PubMed

Šimková, A. , Verneau, O. , Gelnar, M. , & Morand, S. (2006). Specificity and specialization of congeneric monogeneans parasitizing cyprinid fish. Evolution, 60, 1023–1037. 10.1111/j.0014-3820.2006.tb01180.x PubMed DOI

Stefani, F. , Galli, P. , Crosa, G. , Zaccara, S. , & Calamari, D. (2004). Alpine and Apennine barriers determining the differentiation of the rudd (Scardinius erythrophthalmus L.) in the Italian peninsula. Ecology of Freshwater Fish, 13, 168–175. 10.1111/j.1600-0633.2004.00060.x DOI

Štefka, J. , Hypša, V. , & Scholz, T. (2009). Interplay of host specificity and biogeography in the population structure of a cosmopolitan endoparasite: microsatellite study of Ligula intestinalis (Cestoda). Molecular Ecology, 18, 1187–1206. PubMed

Steininger, F. F. , & Rögl, F. (1984). Paleogeography and palinspastic reconstruction of the Neogene of the Mediterranean and Paratethys. In Dixon, J. E. & Robertson, A. H. (Eds.), The geological evolution of the eastern Mediterranean (pp. 659–668). Blackwell.

Sušnik, S. , Snoj, A. , Wilson, I. F. , Mrdak, D. , & Weiss, S. (2007). Historical demography of brown trout (Salmo trutta) in the Adriatic drainage including the putative S. letnica endemic to Lake Ohrid. Molecular Phylogenetics and Evolution, 44, 63–76. 10.1016/j.ympev.2006.08.021 PubMed DOI

Tan, M. , & Armbruster, J. W. (2018). Phylogenetic classification of extant genera of fishes of the order Cypriniformes (Teleostei: Ostariophysi). Zootaxa, 4476, 6–39. 10.11646/zootaxa.4476.1.4 PubMed DOI

Umhang, G. , Grenouillet, F. , Bastid, V. , M’Rad, S. , Balot, B. , Oudni‐M’Rad, M. , & Boué, F. (2018). Investigating the genetic diversity of Echinococchus granulosus sensu stricto with new microsatellites. Parasitology Research, 117, 2743–2755. PubMed

Valdivia, I. M. , Criscione, C. D. , Cárdenas, L. , Durán, C. P. , & Oliva, M. E. (2014). Does a facultative precocious life cycle predispose the marine trematode Proctoeces cf. lintoni to inbreeding and genetic differentiation among host species? International Journal for Parasitology, 44, 183–188. PubMed

van Paridon, B. J. , Colwell, D. D. , Goater, C. P. , & Gilleard, J. S. (2017). Population genetic analysis informs the invasion history of the emerging trematode Dicrocoelium dendriticum into Canada. International Journal for Parasitology, 47, 845–856. 10.1016/j.ijpara.2017.04.006 PubMed DOI

Vanhove, M. P. M. , Briscoe, A. G. , Jorissen, M. W. P. , Littlewood, D. T. J. , & Huyse, T. (2018). The first next‐generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae). BMC Genomics, 19, 520. 10.1186/s12864-018-4893-5 PubMed DOI PMC

Vásquez, A. A. , Lounnas, M. , Sánchez, J. , Alba, A. , Milesi, A. , & Hurtrez‐Bousséz, S. (2016). Genetic and infective diversity of the liver fluke Fasciola hepatica (Trematoda: Digenea) from Cuba. Journal of Helminthology, 90, 719–725. PubMed

Vignon, M. , & Sasal, P. (2010). The use of geometric morphometrics in understanding shape variability of sclerotized haptoral structures of monogeneans (Platyhelminthes) with insights into biogeographic variability. Parasitology International, 59, 183–191. 10.1016/j.parint.2010.01.006 PubMed DOI

Waelbroeck, C. , Labeyrie, L. , Michel, E. , Duplessy, J. C. , McManus, J. F. , Lambeck, K. , Balbon, E. , & Labracherie, M. (2002). Sea‐level and deep water temperature changes derived from benthic foraminifera isotopic records. Quaternary Science Reviews, 21, 295–305. 10.1016/S0277-3791(01)00101-9 DOI

Wagner, B. , & Wilke, T. (2011). Evolutionary and geological history of the Balkan lakes Ohrid and Prespa. Biogeosciences, 8, 995–998.

Zobrazit více v PubMed

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