The ever-growing range of possible applications of nanoparticles requires their mass production. However, there are problems resulting from the prevalent methods of nanoparticle production; physico-chemical routes of nanoparticle synthesis are not very environmentally friendly nor cost-effective. Due to this, the scientific community started exploring new methods of nanoparticle assembly with the aid of biological agents. In this study, ethanolic Vitis vinifera cane extract combined with silver nitrate was used to produce silver nanoparticles. These were subsequently characterized using UV-visible (UV-Vis) spectrometry, transmission electron microscopy, and dynamic light-scattering analysis. The antimicrobial activity of produced nanoparticles was tested against the planktonic cells of five strains of Gram-negative bacterium Pseudomonas aeruginosa (PAO1, ATCC 10145, ATCC 15442, DBM 3081, and DBM 3777). After that, bactericidal activity was assessed using solid medium cultivation. In the end, nanoparticles' inhibitory effect on adhering cells was analyzed by measuring changes in metabolic activity (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay-MTT). Our results confirmed that ethanolic Vitis vinifera cane extract is capable of mediating silver nanoparticle production; synthesis was conducted using 10% of extract and 1 mM of silver nitrate. The silver nanoparticles' Z-average was 68.2 d nm, and their zeta potential was -30.4 mV. These silver nanoparticles effectively inhibited planktonic cells of all P. aeruginosa strains in concentrations less than 5% v/v and inhibited biofilm formation in concentrations less than 6% v/v. Moreover, minimum bactericidal concentration was observed to be in the range of 10-16% v/v. According to the results in this study, the use of wine agriculture waste is an ecological and economical method for the production of silver nanoparticles exhibiting significant antimicrobial properties.

Department of Biotechnology Faculty of Food and Biochemical Technology UCT Prague Technická 5 Dejvice Praha 6 166 28 Prague Czech Republic

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Thakkar K.N., Mhatre S.S., Parikh R.Y. Biological synthesis of metallic nanoparticles. Nanomed. Nanotechnol. Biol. Med. 2010;6:257–262. doi: 10.1016/j.nano.2009.07.002. PubMed DOI

Baptista P.V., McCusker M.P., Carvalho A., Ferreira D.A., Mohan N.M., Martins M., Fernandes A.R. Nano-Strategies to Fight Multidrug Resistant Bacteria—“A Battle of the Titans”. Front. Microbiol. 2018;9:1441. doi: 10.3389/fmicb.2018.01441. PubMed DOI PMC

Kulkarni N., Muddapur U. Biosynthesis of Metal Nanoparticles: A Review. J. Nanotechnol. 2014;354:1–8. doi: 10.1155/2014/510246. DOI

El-Seedi H.R., El-Shabasy R.M., Khalifa S.A.M., Saeed A., Shah A., Shah R., Iftikhar F.J., Abdel-Daim M.M., Omri A., Hajrahand N.H., et al. Metal nanoparticles fabricated by green chemistry using natural extracts: Biosynthesis, mechanisms, and applications. RSC Adv. 2019;9:24539–24559. doi: 10.1039/C9RA02225B. PubMed DOI PMC

Shameli K., Bin Ahmad M., Jaffar Al-Mulla E.A., Ibrahim N.A., Shabanzadeh P., Rustaiyan A., Abdollahi Y., Bagheri S., Abdolmohammadi S., Usman M.S., et al. Green biosynthesis of silver nanoparticles using Callicarpa maingayi stem bark extraction. Molecules. 2012;17:8506–8517. doi: 10.3390/molecules17078506. PubMed DOI PMC

Amini S.M. Preparation of antimicrobial metallic nanoparticles with bioactive compounds. Mater. Sci. Eng. C. 2019;103:109809. doi: 10.1016/j.msec.2019.109809. PubMed DOI

Anshup A., Venkataraman J.S., Subramaniam C., Kumar R.R., Priya S., Kumar T.R.S., Omkumar R.V., John A., Pradeep T. Growth of gold nanoparticles in human cells. Langmuir. 2005;21:11562–11567. doi: 10.1021/la0519249. PubMed DOI

Kowshik M., Deshmukh N., Vogel W., Urban J., Kulkarni S.K., Paknikar K.M. Microbial synthesis of semiconductor CdS nanoparticles, their characterization, and their use in the fabrication of an ideal diode. Biotechnol. Bioeng. 2002;78:583–588. doi: 10.1002/bit.10233. PubMed DOI

Lengke M.F., Fleet M.E., Southam G. Biosynthesis of silver nanoparticles by filamentous cyanobacteria from a silver(I) nitrate complex. Langmuir. 2007;23:2694–2699. doi: 10.1021/la0613124. PubMed DOI

Parveen K., Banse V., Ledwani L. AIP Conference Proceedings. AIP Publishing LLC.; Melville, NY, USA: 2016. Green synthesis of nanoparticles: Their advantages and disadvantages; p. 020048.

Rautaray D., Ahmad A., Sastry M. Biosynthesis of CaCO3 Crystals of Complex Morphology Using a Fungus and an Actinomycete. J. Am. Chem. Soc. 2003;125:14656–14657. doi: 10.1021/ja0374877. PubMed DOI

Shahverdi A.R., Minaeian S., Shahverdi H.R., Jamalifar H., Nohi A.-A. Rapid synthesis of silver nanoparticles using culture supernatants of Enterobacteria: A novel biological approach. Processes Biochem. 2007;42:919–923. doi: 10.1016/j.procbio.2007.02.005. DOI

Raheman F., Deshmukh S., Ingle A., Gade A., Rai M. Silver Nanoparticles: Novel Antimicrobial Agent Synthesized from an Endophytic Fungus Pestalotia sp. Isolated from leaves of Syzygium cumini (L) Nano Biomed. Eng. 2011;3:174–178. doi: 10.5101/nbe.v3i3.p174-178. DOI

Sunkar S., Nachiyar C.V. Biogenesis of antibacterial silver nanoparticles using the endophytic bacterium Bacillus cereus isolated from Garcinia xanthochymus. Asian Pac. J. Trop. Biomed. 2012;2:953–959. doi: 10.1016/S2221-1691(13)60006-4. PubMed DOI PMC

Kora A.J., Sashidhar R.B., Arunachalam J. Aqueous extract of gum olibanum (Boswellia serrata): A reductant and stabilizer for the biosynthesis of antibacterial silver nanoparticles. Processes Biochem. 2012;47:1516–1520. doi: 10.1016/j.procbio.2012.06.004. DOI

Chandran K., Song S., Yun S.-I. Effect of size and shape controlled biogenic synthesis of gold nanoparticles and their mode of interactions against food borne bacterial pathogens. Arab. J. Chem. 2014;12:1994–2006. doi: 10.1016/j.arabjc.2014.11.041. DOI

Raut R.W., Mendhulkar V.D., Kashid S.B. Photosensitized synthesis of silver nanoparticles using Withania somnifera leaf powder and silver nitrate. J. Photochem. Photobiol. B Biol. 2014;132:45–55. doi: 10.1016/j.jphotobiol.2014.02.001. PubMed DOI

Sathishkumar M., Sneha K., Won S.W., Cho C.W., Kim S., Yun Y.S. Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids Surf. B Biointerfaces. 2009;73:332–338. doi: 10.1016/j.colsurfb.2009.06.005. PubMed DOI

Gnanajobitha G., Paulkumar K., Vanaja M., Rajeshkumar S., Malarkodi C., Annadurai G., Kannan C. Fruit-mediated synthesis of silver nanoparticles using Vitis vinifera and evaluation of their antimicrobial efficacy. J. Nanostructure Chem. 2013;3:67. doi: 10.1186/2193-8865-3-67. DOI

Saratale R.G., Saratale G.D., Ahn S., Shin H.-S. Grape Pomace Extracted Tannin for Green Synthesis of Silver Nanoparticles: Assessment of Their Antidiabetic, Antioxidant Potential and Antimicrobial Activity. Polymers. 2021;13:4355. doi: 10.3390/polym13244355. PubMed DOI PMC

Kuppusamy P., Yusoff M.M., Maniam G.P., Govindan N. Biosynthesis of metallic nanoparticles using plant derivatives and their new avenues in pharmacological applications—An updated report. Saudi Pharm. J. 2016;24:473–484. doi: 10.1016/j.jsps.2014.11.013. PubMed DOI PMC

Jacobs C., Kayser O., Müller R.H. Nanosuspensions as a new approach for the formulation for the poorly soluble drug tarazepide. Int. J. Pharm. 2000;196:161–164. doi: 10.1016/S0378-5173(99)00412-3. PubMed DOI

Aruguete D.M., Kim B., Hochella M.F., Ma Y., Cheng Y., Hoegh A., Liu J., Pruden A. Antimicrobial nanotechnology: Its potential for the effective management of microbial drug resistance and implications for research needs in microbial nanotoxicology. Environ. Sci. Process. Impacts. 2012;15:93–102. doi: 10.1039/C2EM30692A. PubMed DOI

Makarov V.V., Love A.J., Sinitsyna O.V., Makarova S.S., Yaminsky I.V., Taliansky M.E., Kalinina N.O. “Green” nanotechnologies: Synthesis of metal nanoparticles using plants. Acta Nat. 2014;6:95. doi: 10.32607/20758251-2014-6-1-35-44. PubMed DOI PMC

Packialakshmi N., Naziya S. Green Synthesis of Silver Nanoparticles from Stem Extracts of Caralluma Fimbriyata and Its Antibacterial Activity. Int. J. Appl. Sci. Biotechnol. 2014;2:305–310. doi: 10.3126/ijasbt.v2i3.10796. DOI

Bharathi D., Diviya Josebin M., Vasantharaj S., Bhuvaneshwari V. Biosynthesis of silver nanoparticles using stem bark extracts of Diospyros montana and their antioxidant and antibacterial activities. J. Nanostruct. Chem. 2018;8:83–92. doi: 10.1007/s40097-018-0256-7. DOI

Haiss W., Thanh N.T.K., Aveyard J., Fernig D.G. Determination of Size and Concentration of Gold Nanoparticles from UV−Vis Spectra. Anal. Chem. 2007;79:4215–4221. doi: 10.1021/ac0702084. PubMed DOI

Nayak D., Ashe S., Rauta P.R., Kumari M., Nayak B. Bark extract mediated green synthesis of silver nanoparticles: Evaluation of antimicrobial activity and antiproliferative response against osteosarcoma. Mater. Sci. Eng. C. 2016;58:44–52. doi: 10.1016/j.msec.2015.08.022. PubMed DOI

Rao B., Tang R.-C. Green synthesis of silver nanoparticles with antibacterial activities using aqueous Eriobotrya japonica leaf extract. Adv. Nat. Sci. Nanosci. Nanotechnol. 2017;8:015014. doi: 10.1088/2043-6254/aa5983. DOI

Ravichandran V., Vasanthi S., Shalini S., Ali Shah S.A., Harish R. Green synthesis of silver nanoparticles using Atrocarpus altilis leaf extract and the study of their antimicrobial and antioxidant activity. Mater. Lett. 2016;180:264–267. doi: 10.1016/j.matlet.2016.05.172. DOI

Anandalakshmi K., Venugobal J., Ramasamy V. Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity. Appl. Nanosci. 2016;6:399–408. doi: 10.1007/s13204-015-0449-z. DOI

Singh P., Pandit S., Garnæs J., Tunjic S., Mokkapati V.R.S.S., Sultan A., Thygesen A., Mackevica A., Mateiu R.V., Daugaard A.E., et al. Green synthesis of gold and silver nanoparticles from Cannabis sativa (industrial hemp) and their capacity for biofilm inhibition. Int. J. Nanomed. 2018;13:3571–3591. doi: 10.2147/IJN.S157958. PubMed DOI PMC

Sadeghi B., Gholamhoseinpoor F. A study on the stability and green synthesis of silver nanoparticles using Ziziphora tenuior (Zt) extract at room temperature. Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 2015;134:310–315. doi: 10.1016/j.saa.2014.06.046. PubMed DOI

Ahmed S., Saifullah, Ahmad M., Swami B.L., Ikram S. Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. J. Radiat. Res. Appl. Sci. 2016;9:1–7. doi: 10.1016/j.jrras.2015.06.006. DOI

Vijayakumar A.S. Pharmacological activity of silver nanoparticles, ethanolic extract from justicia gendarussa (burm) f plant leaves. Res. J. Life Sci. Bioinform. Pharm. Chem. Sci. 2019;5:462–475. doi: 10.26479/2019.0502.33. DOI

Deshmukh S.P., Patil S.M., Mullani S.B., Delekar S.D. Silver nanoparticles as an effective disinfectant: A review. Mater. Sci. Eng. C. 2019;97:954–965. doi: 10.1016/j.msec.2018.12.102. PubMed DOI PMC

Vaňková E., Paldrychová M., Kašparová P., Lokočová K., Kodeš Z., Maťátková O., Kolouchová I., Masák J. Natural antioxidant pterostilbene as an effective antibiofilm agent, particularly for gram-positive cocci. World J. Microbiol. Biotechnol. 2020;36:101. doi: 10.1007/s11274-020-02876-5. PubMed DOI

Pompilio A., Geminiani C., Bosco D., Rana R., Aceto A., Bucciarelli T., Scotti L., Di Bonaventura G. Electrochemically Synthesized Silver Nanoparticles Are Active Against Planktonic and Biofilm Cells of Pseudomonas aeruginosa and Other Cystic Fibrosis-Associated Bacterial Pathogens. Front. Microbiol. 2018;9:1349. doi: 10.3389/fmicb.2018.01349. PubMed DOI PMC

Singh K., Panghal M., Kadyan S., Chaudhary U., Yadav J.P. Green silver nanoparticles of Phyllanthus amarus: As an antibacterial agent against multi drug resistant clinical isolates of Pseudomonas aeruginosa. J. Nanobiotechnol. 2014;12:40. doi: 10.1186/s12951-014-0040-x. PubMed DOI PMC

Radzig M., Koksharova O., Khmel I.A. Antibacterial effects of silver ions: Effect on gram-negative bacteria growth and biofilm formation. Mol. Gen. Mikrobiol. Virusol. 2009;24:194–199. doi: 10.3103/S0891416809040065. PubMed DOI

Palanisamy N.K., Ferina N., Amirulhusni A.N., Mohd-Zain Z., Hussaini J., Ping L.J., Durairaj R. Antibiofilm properties of chemically synthesized silver nanoparticles found against Pseudomonas aeruginosa. J. Nanobiotechnol. 2014;12:2. doi: 10.1186/1477-3155-12-2. PubMed DOI PMC

Markowska K., Grudniak A.M., Krawczyk K., Wróbel I., Wolska K.I. Modulation of antibiotic resistance and induction of a stress response in Pseudomonas aeruginosa by silver nanoparticles. J. Med. Microbiol. 2014;63:849–854. doi: 10.1099/jmm.0.068833-0. PubMed DOI

Lara H., Ayala-Nunez V., Ixtepan Turrent L., Rodríguez-Padilla C. Bactericidal effect of AgNPs against multidrug-resistant bacteria. World J. Microbiol. Biotechnol. 2009;26:615–621. doi: 10.1007/s11274-009-0211-3. DOI

Mohan S., Oluwafemi O.S., George S.C., Jayachandran V.P., Lewu F.B., Songca S.P., Kalarikkal N., Thomas S. Completely green synthesis of dextrose reduced silver nanoparticles, its antimicrobial and sensing properties. Carbohydr. Polym. 2014;106:469–474. doi: 10.1016/j.carbpol.2014.01.008. PubMed DOI

Rollová M., Gharwalova L., Krmela A., Schulzová V., Hajšlová J., Jaroš P., Kolouchová I., Maťátková O. Grapevine extracts and their effect on selected gut-associated microbiota: In vitro study. Czech. J. Food Sci. 2020;38:137–143. doi: 10.17221/308/2019-CJFS. DOI

Sharma M., Manoharlal R., Negi A.S., Prasad R. Synergistic anticandidal activity of pure polyphenol curcumin I in combination with azoles and polyenes generates reactive oxygen species leading to apoptosis. FEMS Yeast Res. 2010;10:570–578. doi: 10.1111/j.1567-1364.2010.00637.x. PubMed DOI

Serra E., Hidalgo-Bastida L.A., Verran J., Williams D., Malic S. Antifungal Activity of Commercial Essential Oils and Biocides against Candida albicans. Pathogens. 2018;7:15. doi: 10.3390/pathogens7010015. PubMed DOI PMC

Rahal J.J., Simberkoff M.S. Bactericidal and Bacteriostatic Action of Chloramphenicol Against Meningeal Pathogens. Antimicrob. Agents Chemother. 1979;16:13–18. doi: 10.1128/AAC.16.1.13. PubMed DOI PMC

Shin D.-S., Eom Y.-B. Zerumbone inhibits Candida albicans biofilm formation and hyphal growth. Can. J. Microbiol. 2019;65:713–721. doi: 10.1139/cjm-2019-0155. PubMed DOI

Sabaeifard P., Abdi-Ali A., Soudi M.R., Dinarvand R. Optimization of tetrazolium salt assay for Pseudomonas aeruginosa biofilm using microtiter plate method. J. Microbiol. Methods. 2014;105:134–140. doi: 10.1016/j.mimet.2014.07.024. PubMed DOI

Exploring the antimicrobial potential of chitosan nanoparticles: synthesis, characterization and impact on Pseudomonas aeruginosa virulence factors