Degradation of undesirable biogenic amines (BAs) in foodstuffs by microorganisms is considered one of the most effective ways of eliminating their toxicity. In this study, we design two sets of primers for the detection and quantification of the amine oxidase gene (yobN) and endogenous (housekeeping) gene (gyrB) in Bacillus subtilis. Moreover, these sets can be used for relative quantification of yobN by real-time PCR (qPCR). We also tested the degradation of BAs by three bacterial strains (B. subtilis strains: IB1a, CCM 2216, CCM 2267) in a mineral medium over a two-day period. Their degradation abilities were verified by high performance liquid chromatography with UV detection (HPLC/UV). According to the results, two strains significantly (P < 0.05) reduced histamine, tyramine, putrescine, and cadaverine. Moreover, our results indicate that the degradation ability of B. subtilis strains could be limited by sporulation because the gene encoding amine oxidase (yobN) is no longer expressed in the spores.

Centre of Polymer Systems University Institute Tomas Bata University in Zlín Tř T Bati 5678 760 01 Zlín Czech Republic

Department of Environmental Protection Engineering Faculty of Technology Tomas Bata University in Zlín Vavrečkova 275 76001 Zlín Czech Republic

Department of Food Technology Faculty of Technology Tomas Bata University in Zlín Vavrečkova 275 76001 Zlín Czech Republic

Faculty of Military Leadership University of Defence in Brno Kounicova 65 662 10 Brno Czech Republic

University Institute Tomas Bata University in Zlín Nad Ovčírnou 8685 760 01 Zlín Czech Republic

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