BACKGROUND: Intravascular delivery of nanoparticles for theranostic application permits direct interaction of nanoparticles and vascular cells. Since vascular smooth muscle cells (VSMCs), the major components of the vascular wall, are constantly subjected to mechanical stimulation from hemodynamic influence, we asked whether cyclic strain may modulate internalization of magnetic nanoparticles (MNPs) by cultured VSMCs. METHODS: Cyclic strain (1 Hz and 10%) was applied with Flexcell system in cultured VSMCs from rats, with cell-associated MNPs (MNPcell) determined by a colorimetric iron assay. Transmission and scanning electron microscopy were used for morphology studies. Confocal microscopy was used to demonstrate distribution of actin assembly in VSMCs. RESULTS: Incubation of poly(acrylic acid) (PAA)-coated MNPs with VSMCs for 4 h induced microvilli formation and MNP internalization. Application of cyclic strain for 4-12 h significantly reduced MNPcell by up to 65% (p < 0.05), which was associated with blunted microvilli and reduced vesicle size/cell, but not vesicle numbers/cell. Confocal microscopy demonstrated that both cyclic strain and fibronectin coating of the culture plate reduced internalized MNPs, which were co-localized with vinculin. Furthermore, cytochalasin D reduced MNPcell, suggesting a role of actin polymerization in MNP uptake by VSMCs; however, a myosin II ATPase inhibitor, blebbistatin, exhibited no effect. Cyclic strain also attenuated uptake of PAA-MNPs by LN-229 cells and uptake of poly-L-lysine-coated MNPs by VSMCs. CONCLUSION: In such a dynamic milieu, cyclic strain may impede cellular internalization of nanocarriers, which spares the nanocarriers and augments their delivery to the target site in the lumen of vessels or outside of the circulatory system.

Department of Biochemistry and Molecular Biology College of Medicine Chang Gung University Taoyuan 33302 Taiwan Republic of China

Department of Medical Imaging and Intervention Chang Gung Memorial Hospital Taoyuan 33305 Taiwan Republic of China

Department of Physiology and Pharmacology Chang Gung University Taoyuan 33302 Taiwan Republic of China

Institute of Biomedical Sciences Chang Gung University Taoyuan 33302 Taiwan Republic of China

Institute of Macromolecular Chemistry Academy of Sciences of the Czech Republic Prague 6 162 06 Czech Republic

Liver Research Center Chang Gung Memorial Hospital Taoyuan 33305 Taiwan Republic of China

Zobrazit více v PubMed

Panyam J, Labhasetwar V. Dynamics of endocytosis and exocytosis of poly(D,L-lactide-co-glycolide) nanoparticles in vascular smooth muscle cells. PubMed DOI

Freese C, Schreiner D, Anspach L, et al. In vitro investigation of silica nanoparticle uptake into human endothelial cells under physiological cyclic stretch. PubMed DOI PMC

Lu YC, Luo PC, Huang CW, et al. Augmented cellular uptake of nanoparticles using tea catechins: effect of surface modification on nanoparticle-cell interaction. PubMed DOI

Kakisis JD, Liapis CD, Sumpio BE. Effects of cyclic strain on vascular cells. PubMed DOI

Su BY, Shontz KM, Flavahan NA, Nowicki PT. The effect of phenotype on mechanical stretch-induced vascular smooth muscle cell apoptosis. PubMed DOI

Ando J, Yamamoto K. Effects of shear stress and stretch on endothelial function. PubMed DOI

Fang Y, Wu D, Birukov KG. Mechanosensing and mechanoregulation of endothelial cell functions. PubMed PMC

van Engeland NCA, Pollet A, den Toonder JMJ, Bouten CVC, Stassen O, Sahlgren CM. A biomimetic microfluidic model to study signalling between endothelial and vascular smooth muscle cells under hemodynamic conditions. PubMed DOI PMC

Jufri NF, Mohamedali A, Avolio A, Baker MS. Mechanical stretch: physiological and pathological implications for human vascular endothelial cells. PubMed DOI PMC

Lim CG, Jang J, Kim C. Cellular machinery for sensing mechanical force. PubMed DOI PMC

Rensen SS, Doevendans PA, van Eys GJ. Regulation and characteristics of vascular smooth muscle cell phenotypic diversity. PubMed DOI PMC

Chen J, Zhou Y, Liu S, Li C. Biomechanical signal communication in vascular smooth muscle cells. PubMed DOI PMC

Hu X, Zhao P, Lu Y, Liu Y. ROS-based nanoparticles for atherosclerosis treatment. PubMed DOI PMC

Behzadi S, Serpooshan V, Tao W, et al. Cellular uptake of nanoparticles: journey inside the cell. PubMed DOI PMC

Oh N, Park JH. Endocytosis and exocytosis of nanoparticles in mammalian cells. PubMed PMC

Zhang S, Gao H, Bao G. Physical principles of nanoparticle cellular endocytosis. PubMed DOI PMC

Yameen B, Choi WI, Vilos C, Swami A, Shi J, Farokhzad OC. Insight into nanoparticle cellular uptake and intracellular targeting. PubMed DOI PMC

Swanson JA. Shaping cups into phagosomes and macropinosomes. PubMed DOI PMC

Mooren OL, Galletta BJ, Cooper JA. Roles for actin assembly in endocytosis. PubMed DOI

Gauthier NC, Masters TA, Sheetz MP. Mechanical feedback between membrane tension and dynamics. PubMed DOI

Carlsson AE. Membrane bending by actin polymerization. PubMed DOI PMC

Blanchoin L, Boujemaa-Paterski R, Sykes C, Plastino J. Actin dynamics, architecture, and mechanics in cell motility. PubMed DOI

Gallop JL. Filopodia and their links with membrane traffic and cell adhesion. PubMed DOI

Mogilner A. On the edge: modeling protrusion. PubMed DOI

Ohashi K, Fujiwara S, Mizuno K. Roles of the cytoskeleton, cell adhesion and rho signalling in mechanosensing and mechanotransduction. PubMed

Bauer MS, Baumann F, Daday C, et al. Structural and mechanistic insights into mechanoactivation of focal adhesion kinase. PubMed DOI PMC

Wrighton KH. Cell adhesion: the ‘ins’ and ‘outs’ of integrin signalling. PubMed

Xiao Y, Du J. Superparamagnetic nanoparticles for biomedical applications. PubMed DOI

Revia RA, Zhang M. Magnetite nanoparticles for cancer diagnosis, treatment, and treatment monitoring: recent advances. PubMed DOI PMC

Ulbrich K, Holá K, Šubr V, Bakandritsos A, Tuček J, Zbořil R. Targeted drug delivery with polymers and magnetic nanoparticles: covalent and noncovalent approaches, release control, and clinical studies. PubMed

Siow WX, Chang YT, Babič M, Lu YC, Horák D, Ma YH. Interaction of poly-l-lysine coating and heparan sulfate proteoglycan on magnetic nanoparticle uptake by tumor cells. PubMed DOI PMC

Dabaghi M, Hilger I. Magnetic nanoparticles behavior in biological solutions; the impact of clustering tendency on sedimentation velocity and cell uptake. PubMed DOI PMC

Rouse JG, Haslauer CM, Loboa EG, Monteiro-Riviere NA. Cyclic tensile strain increases interactions between human epidermal keratinocytes and quantum dot nanoparticles. PubMed DOI

Ma YH, Wei HW, Su KH, Ives HE, Morris RC. Chloride-dependent calcium transients induced by angiotensin II in vascular smooth muscle cells. PubMed DOI

Lu YC, Chang FY, Tu SJ, Chen JP, Ma YH. Cellular uptake of magnetite nanoparticles enhanced by NdFeB magnets in staggered arrangement. DOI

Casella JF, Flanagan MD, Lin S. Cytochalasin D inhibits actin polymerization and induces depolymerization of actin filaments formed during platelet shape change. PubMed DOI

Kovács M, Tóth J, Hetényi C, Málnási-Csizmadia A, Sellers JR. Mechanism of blebbistatin inhibition of myosin II. PubMed DOI

Sakulkhu U, Mahmoudi M, Maurizi L, Salaklang J, Hofmann H. Protein Corona composition of superparamagnetic iron oxide nanoparticles with various physico-chemical properties and coatings. PubMed DOI PMC

Kokkinopoulou M, Simon J, Landfester K, Mailänder V, Lieberwirth I. Visualization of the protein Corona: towards a biomolecular understanding of nanoparticle-cell-interactions. PubMed DOI

Chiu CY, Chung TW, Chen SY, Ma YH. Effects of PEGylation on capture of dextran-coated magnetic nanoparticles in microcirculation. PubMed PMC

Raucher D, Sheetz MP. Cell spreading and lamellipodial extension rate is regulated by membrane tension. PubMed DOI PMC

Batchelder EL, Hollopeter G, Campillo C, et al. Membrane tension regulates motility by controlling lamellipodium organization. PubMed DOI PMC

Hu J, Liu Y. Cyclic strain enhances cellular uptake of nanoparticles.

Freese C, Anspach L, Deller RC, et al. Gold nanoparticle interactions with endothelial cells cultured under physiological conditions. PubMed DOI

Nozumi M, Nakatsu F, Katoh K, Igarashi M. Coordinated movement of vesicles and actin bundles during nerve growth revealed by superresolution microscopy. PubMed DOI

Onishi K, Shafer B, Lo C, Tissir F, Goffinet AM, Zou Y. Antagonistic functions of Dishevelleds regulate Frizzled3 endocytosis via filopodia tips in Wnt-mediated growth cone guidance. PubMed DOI PMC

Bu W, Chou AM, Lim KB, Sudhaharan T, Ahmed S. The Toca-1-N-WASP complex links filopodial formation to endocytosis. PubMed DOI PMC

Dent EW, Kwiatkowski AV, Mebane LM, et al. Filopodia are required for cortical neurite initiation. PubMed DOI

Young LE, Heimsath EG, Higgs HN. Cell type-dependent mechanisms for formin-mediated assembly of filopodia. PubMed DOI PMC

Le Roux AL, Quiroga X, Walani N, Arroyo M, Roca-Cusachs P. The plasma membrane as a mechanochemical transducer. PubMed DOI PMC

Qi YX, Han Y, Jiang ZL. Mechanobiology and vascular remodeling: from membrane to nucleus. PubMed

Li W, Sancho A, Chung WL, et al. Differential cellular responses to adhesive interactions with galectin-8- and fibronectin-coated substrates. PubMed DOI PMC

Chazotte B. Labeling membrane glycoproteins or glycolipids with fluorescent wheat germ agglutinin. PubMed DOI

Dhein S, Schreiber A, Steinbach S, et al. Mechanical control of cell biology. Effects of cyclic mechanical stretch on cardiomyocyte cellular organization. PubMed DOI

Impact of mechanical cues on key cell functions and cell-nanoparticle interactions