Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
35315940
DOI
10.1002/elps.202100379
Knihovny.cz E-zdroje
- Klíčová slova
- bioanalysis, hemoglobin, mass spectrometry, microfluidics, single-cell analysis,
- MeSH
- erytrocyty MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací * metody MeSH
- laboratoř na čipu MeSH
- lidé MeSH
- mikrofluidika * metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Analysis of cellular composition and metabolism at a single-cell resolution allows gaining more information about complex relationships of cells within tissues or whole living organisms by resolving the variance stemming from the cellular heterogeneity. Mass spectrometry (MS) is a perfect analytical tool satisfying the demanding requirements of detecting and identifying compounds present in such ultralow-volume samples of high chemical complexity. However, the method of sampling and sample ionization is crucial in obtaining relevant information. In this work, we present a microfluidic sampling platform that integrates single-cell extraction from MS-incompatible media with electrical cell lysis and nanoESI-MS analysis of human erythrocytes. Hemoglobin alpha and beta chains (300 amol/cell) were successfully identified in mass spectra of single-erythrocyte lysates.
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