In an effort to expand knowledge of Clade 3-one of the ten clades that compose the non-monophyletic order 'Tetraphyllidea' all current members of which parasitise orectolobiform sharks-we targeted species of orectolobiform sharks that had not previously been examined for 'tetraphyllidean' cestodes. That work led to the discovery of three new species off Australia and Taiwan. Ambitalveolus gen. n. was erected to accommodate these species. Ambitalveolus costelloae gen. n. et sp. n., Ambitalveolus kempi sp. n., and Ambitalveolus penghuensis sp. n. differ from one another in scolex size, genital pore position, and number of marginal loculi, proglottids, and testes. Among 'tetraphyllideans', the new genus most closely resembles the two other genera in Clade 3. It differs from Carpobothrium Shipley et Hornell, 1906 in lacking anterior and posterior flap-like extensions of its bothridia; instead, its bothridia are essentially circular. It differs from Caulopatera Cutmore, Bennett et Cribb, 2010 in that its vitelline follicles are in two lateral bands, rather than circum-medullary, and in that its bothridia bear, rather than lack, conspicuous marginal loculi. A key to the three genera in Clade 3 is provided. A phylogenetic analysis including new sequence data for one of the three new species of Ambitalveolus gen. n., the only species of Caulopatera, and all four described species and one undescribed species of Carpobothrium supports previously hypothesised close affinities between Caulopatera and Carpobothrium, with the new genus as their sister group. This is the first report of 'tetraphyllidean' cestodes from the orectolobiform shark family Brachaeluridae Applegate. The association of the new species with orectolobiform sharks is consistent with those of the other members of Clade 3. However, whereas species of Carpobothrium and Caulopatera parasitise members of the hemiscylliid genus Chiloscyllium Müller et Henle, species of Amitalveolus gen. n. parasitise members of the Brachaeluridae and Orectolobidae Gill.

Department of Ecology and Evolutionary Biology and the Biodiversity Institute University of Kansas Lawrence Kansas USA

Department of Ecology and Evolutionary Biology University of Connecticut Storrs Connecticut USA

Zobrazit více v PubMed

Baer J.G., Euzet L. 1962: Revision critique des cestodes tétraphyllides décrits par T. Southwell (1re partie). Bull. Soc. Neuchatel Sci. Nat. 85: 143-172.

Butler S.A. 1987: Taxonomy of some tetraphyllidean cestodes from elasmobranch fishes. Aust. J. Zool. 35: 343-371. DOI

Caira J.N. 1992: Verification of multiple species of Pedibothrium in the Atlantic nurse shark with comments on the Australasian members of the genus. J. Parasitol. 78: 289-308. DOI

Caira J.N., Euzet L. 2001: Age of association between the nurse shark, Ginglymostoma cirratum, and tapeworms of the genus Pedibothrium (Tetraphyllidea: Onchobothriidae): implications from geography. Biol. J. Linn. Soc. 72: 609-614. DOI

Caira J.N., Jensen K., Barbeau E. (Eds.) 2021a: Global Cestode Database. World Wide Web electronic publication, https://tapewormdb.uconn.edu/.

Caira J.N., Jensen K., Hayes C., Ruhnke T.R. 2020: Insights from new cestodes of the crocodile shark, Pseudocarcharias kamoharai (Lamniformes: Pseudocarchariidae), prompt expansion of Scyphyophyllidum and formal synonymization of seven phyllobothriidean genera - at last! J. Helminthol. 94: e132. PubMed DOI

Caira J.N., Jensen K., Healy C.J. 1999: On the phylogenetic relationships among tetraphyllidean, lecanicephalidean and diphyllidean tapeworm genera. Syst. Parasitol. 42: 77-151. PubMed DOI

Caira J.N., Jensen K., Healy C.J. 2001: Interrelationships among tetraphyllidean and lecanicephalidean cestodes. In: D.T.J. Littlewood and R.A. Bray (Eds.), Interrelationships of the Platyhelminthes. Taylor & Francis, London, pp. 135-158.

Caira J.N., Jensen K., Ruhnke T.R. 2017: "Tetraphyllidea" van Beneden, 1849 relics. In: J.N. Caira and K. Jensen (Eds.), Planetary Biodiversity Inventory (2008-2017): Tapeworms from Vertebrate Bowels of the Earth. University of Kansas, Natural History Museum, Special Publication No. 25, Lawrence, pp. 371-400.

Caira J.N., Jensen K., Waeschenbach A., Olson P.D., Littlewood D.T.J. 2014: Orders out of chaos-molecular phylogenetics reveals the complexity of shark and stingray tapeworm relationships. Int. J. Parasitol. 44: 55-73. DOI

Caira J.N., Otto K., Fernando D., Jensen K. 2021b: Three new species of 'tetraphyllidean' cestodes from an undescribed bamboo shark (Orectolobiformes: Hemiscylliidae) in Sri Lanka. Folia Parasitol. 68: 004. DOI

Caira J.N., Rasolofonirina R. 1998: A new species of Pedibothrium (Cestoidea: Tetraphyllidea) from the short-tail nurse shark, Pseudoginglymostoma brevicaudatum (Elasmobranchii: Orectilobiformes), from southwest Madagascar. J. Helm. Soc. Wash. 65: 50-55.

Caira J.N., Tracy R., Euzet L. 2004: Five new species of Pedibothrium (Tetraphyllidea: Onchobothriidae) from the tawny nurse shark, Nebrius ferrugineus, in the Pacific Ocean. J. Parasitol. 90: 286-300. PubMed DOI

Campbell R.A., Beveridge I. 2002: The genus Acanthobothrium (Cestoda: Tetraphyllidea: Onchobothriidae) parasitic in Australian elasmobranch fishes. Invertebr. Syst. 16: 237-344. DOI

Chervy L. 2009: Unified terminology for cestode microtriches: a proposal from the international workshops on cestode systematics in 2002-2008. Folia Parasitol. 56: 199-230. DOI

Cutmore S.C., Bennett M.B., Cribb T.H. 2010: A new tetraphyllidean genus and species, Caulopatera pagei n. g., n. sp. (Tetraphyllidea: Phyllobothriidae), from the grey carpetshark Chiloscyllium punctatum Müller & Henle (Orectolobiformes: Hemiscylliidae). Syst. Parasitol. 77: 13-21. PubMed DOI

Cutmore S.C., Bennett M.B., Miller T.L., Cribb T.H. 2017: Patterns of specificity and diversity in species of Paraorygmatobothrium Ruhnke, 1994 (Cestoda: Phyllobothriidae) in Moreton Bay, Queensland, Australia, with the description of four new species. Syst. Parasitol. 94: 941-970. PubMed DOI

Cutmore S.C., Cribb T.H., Bennett M.B., Beveridge I. 2018: Tetraphyllidean and onchoproteocephalidean cestodes of elasmobranchs from Moreton Bay, Australia: description of two new species and new records for seven described species. Syst. Parasitol. 95: 807-827. PubMed DOI

Darriba D., Taboada G.L., Doallo R., Posada D. 2012: jModelTest 2: more models, new heuristics and parallel computing. Nat. Meth. 9: 772. DOI

Deshmukh R.A. 1979: On a new cestode Yorkeria southwelli (Cestoda: Onchobothriidae) from a marine fish. Current Science 48: 271-272.

Deshmukh R.A., Shinde G.B. 1980: Spinibiloculus ratnagiriensis gen. n., sp. n. (Cestoda, Onchobothriidae) from a marine fish Ginglymostoma concolor of the west coast of India. Acta Parasitol. 27: 431-435.

Eudy E., Caira J.N., Jensen K. 2019: A new species of Pentaloculum (Cestoda: "Tetraphyllidea) from the Taiwan saddled carpetshark, Cirrhoscyllium formosanum (Orectolobiformes: Parascylliidae). J. Parasitol. 105: 303-312. PubMed DOI

Fernando D., Bown R.M.K., Tanna A., Gobiraj R., Ralicki H., Jockusch E.L., Ebert D.A., Jensen K., Caira J.N. 2019: New insights into the identities of the elasmobranch fauna of Sri Lanka. Zootaxa 4585: 201-238. DOI

Fyler C.A. 2011: An extremely hyperapolytic Acanthobothrium species (Cestoda: Tetraphyllidea) from the Japanese wobbegong, Orectolobus japonicus (Elasmobranchii: Orectolobiformes) in Taiwan. Comp. Parasitol. 78: 4-14. DOI

Healy C.J., Caira J.N., Jensen K., Webster B.L., Littlewood D.T.J. 2009: Proposal for a new tapeworm order, Rhinebothriidea. Int. J. Parasitol. 39: 497-511. DOI

Hornell J. 1912: New cestodes from Indian fishes. Rec. Indian Mus. 7: 197-204. DOI

Jensen K., Pen I.A.M., Caira J.N. 2021: A revision of the Rhoptrobothriidae (Cestoda: "Tetraphyllidea). Zootaxa 4999: 201-218. PubMed DOI

Khambata F.S., Bal D.V. 1954: Studies on the cestodes of marine fishes from Bombay. Part I - On the genus Balanobothrium Hornell, 1912 (Cestoda), with a description of a new species. J. Zool. Soc. India 6: 101-110.

Koontz A., Caira J.N. 2016: Emendation of Carpobothrium (Tetraphyllidea) from bamboosharks (Orectolobiformes: Hemiscyliidae [sic]) with redescription of Carpobothrium chiloscyllii and description of a new species from Borneo. Comp. Parasitol. 83: 149-161. DOI

Löytynoja A., Goldman N. 2010: webPRANK: a phylogeny-aware multiple sequence aligner with interactive alignment browser. BMC Bioinformatics 11: 579. PubMed DOI

Naylor G.J.P., Caira J.N., Jensen K., Rosana K.A.M., Straube N., Lakner C. 2012: Elasmobranch phylogeny: a mitochondrial estimate based on 595 species. In: J.C. Carrier, J.A. Musick and M.R. Heithaus (Eds.), The Biology of Sharks and Their Relatives. CRC Press, Taylor & Francis Group, Boca Raton, pp. 31-56. DOI

Pleijel F., Jondelius U., Norlinder E., Nygren A., Oxelman B., Schander C., Sundberg P., Thollesson M. 2008: Phylogenies without roots? A plea for the use of vouchers in molecular phylogenetic studies. Mol. Phylogenet. Evol. 48: 369-371. PubMed DOI

Shinde G.B., Jadhav B.V., Deshmukh R.A. 1980: Two new species of the genus Pedibothrium Linton, 1909 (Cestoda: Oncobothriidae). Proc. Ind. Acad. Parasitol. 1: 21-24.

Southwell T. 1911: Description of nine new species of cestode parasites, including two new genera from marine fishes of Ceylon. Ceylon Mar. Biol. Rep. 1: 216-225.

Sukumaran J., Holder M.T. 2010: DendroPy: A Python library for phylogenetic computing. Bioinformatics 26: 1569-1571. PubMed DOI

Sukumaran J., Holder M.T. 2015. SumTrees: Phylogenetic Tree Summarization. 4.0.0 (31 January 2015).

Williams H.H. 1962: Acanthobothrium sp. nov. (Cestoda: Tetraphyllidea) and a comment on the order Biporophyllaeidea. Parasitology 52: 67-76. DOI

Yamaguti S. 1954: Parasitic worms mainly from Celebes. Part 6. Cestodes of fishes. Acta Med. Okayama 8: 353-374.

Zwickl D.J. 2006. Genetic algorithm approaches for the phylogenetic analysis of large biological sequence datasets under the maximum likelihood criterion. PhD Dissertation. The University of Texas at Austin, Austin, 115 pp.

Fish tapeworms (Cestoda) in the molecular era: achievements, gaps and prospects