Fructooligosaccharides (FOS) are fructose-based oligosaccharides employed as additives to improve the food's nutritional and technological properties. The rhizosphere of plants that accumulate fructopolysaccharides as inulin has been revealed as a source of filamentous fungi. These fungi can produce FOS either by inulin hydrolysis or by biosynthesis from sucrose, including unusual FOS with enhanced prebiotic properties. Here, we investigated the ability of Fusarium solani and Neocosmospora vasinfecta to produce FOS from different carbon sources. Fusarium solani and N. vasinfecta grew preferentially in inulin instead of sucrose, resulting in the FOS production as the result of endo-inulinase activities. N. vasinfecta was also able to produce the FOS 1-kestose and 6-kestose from sucrose, indicating transfructosylating activity, absent in F. solani. Moreover, the results showed how these carbon sources affected fungal cell wall composition and the expression of genes encoding for β-1,3-glucan synthase and chitin synthase. Inulin and fructose promoted changes in fungal macroscopic characteristics partially explained by alterations in cell wall composition. However, these alterations were not directly correlated with the expression of genes related to cell wall synthesis. Altogether, the results pointed to the potential of both F. solani and N. vasinfecta to produce FOS at specific profiles.

Departamento de Genética Universidade Federal de Pernambuco Av Prof Moraes Rego 1235 50670 901 Recife PE Brazil

Faculdade da Saúde Universidade Metodista de São Paulo Rua Alfeu Tavares 149 Sao Bernardo do Campo SP 09641 000 Brazil

Instituto de Ciências Biológicas Universidade de Pernambuco R Arnóbio Marques 310 50100 130 Recife PA Brazil

Núcleo de Conservação da Biodiversidade Instituto de Pesquisas Ambientais Av Miguel Stéfano 3687 São Paulo SP 04301 902 Brazil

Programa de Pós Graduação em Biologia Celular e Estrutural Universidade Estadual de Campinas Campinas SP Brazil

Unidade Acadêmica de Garanhuns Universidade Federal Rural de Pernambuco R Bom Pastor s n 55 292 270 Garanhuns PA Brazil

Universidade Federal do ABC Avenida dos Estados Santo André SP 5001 09210 580 Brazil

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