Pedomonas mirosovicensis gen. nov., sp. nov., a bacterium isolated from soil with the aid of Micrococcus luteus culture supernatant containing resuscitation-promoting factor
Language English Country England, Great Britain Media print
Document type Journal Article
- Keywords
- Sphingosinicellaceae, compost soil, extracellular organic matter, resuscitation-promoting factor, soil bacteria, whole genome average nucleotide identity,
- MeSH
- Alphaproteobacteria * genetics MeSH
- DNA, Bacterial genetics MeSH
- Phospholipids chemistry MeSH
- Phylogeny MeSH
- Fatty Acids chemistry MeSH
- Micrococcus luteus * MeSH
- Soil MeSH
- Soil Microbiology MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Bacterial Typing Techniques MeSH
- Ubiquinone chemistry MeSH
- Base Composition MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Phospholipids MeSH
- Fatty Acids MeSH
- Soil MeSH
- RNA, Ribosomal, 16S MeSH
- Ubiquinone MeSH
An orange-golden iridescent culture, designated A1X5R2T, was isolated from a compost soil suspension which was amended with Micrococcus luteus NCTC 2665T culture supernatant. The cells were non-motile, Gram-stain-negative, 0.4-0.5 µm wide and 0.7-1.4 µm long. The 16S rRNA-based phylogenetic and whole-genome analyses revealed that strain A1X5R2T forms a distinct lineage within the family Sphingosinicellaceae and is closely related to members of the genus Sphingoaurantiacus (S. capsulatus, 93.04 % similarity, and S. polygranulatus, 92.77 %). The organism grew at 22-47 °C (optimal at 37 °C), salinity <3 % (optimal at 1.5 %) and at pH 7. The major respiratory quinone was ubiquinone-10, but a small quantity of ubiquinone-9 was also detected The major polyamine was homospermidine, but a small quantity of putrescine was also detected. The strain contained C18 : 1ω7c, C16 : 0, C16 : 1 ω7c and C18 : 0 as the major fatty acids. The main polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, sphingoglycolipid, diphosphatidylglycerol, two unidentified phospholipids and three unidentified amino lipids. The DNA G+C content was 64.9 mol%. According to the results of phylogenetic and phylogenomic analyses, as well as its physiological characteristics, strain A2X5R2T represents the type species of a novel genus within the family Sphingosinicellaceae. The name Pedomonas mirosovicensis gen. nov., sp. nov. is proposed, with the type strain being A1X5R2T (=NCCB 100839T=DSM 112829T).
Institute of Microbiology of the Czech Academy of Sciences Videnska 1083 14220 Prague Czech Republic
References provided by Crossref.org
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